A couple of questions then...

Water in the loaded acetone, yes or no?

Defatting before acetone pull, yes or no? If yes for last one, which nonpolar to use without pulling freebase bufotenine?

My latest attempt with 10 g is yielding more than before (3%), this time it was a very dry loaded acetone that I added a droplet of water and put it in the freezer. Got larger coagulates/precipitants instead of powder (excluding the fats that make white precipitates, but dissolve back at room temp). Next time I will split into tubes and try different things before adding acid like pre-drying, with and without amounts of water and so on.