acacia floribunda rootbark extraction

[acacian]

hi all
a mate is currently doing a quick stb extraction on 30g acacia floribunda fresh rootbark. was bright red in the inner layer and light grey on the outer layer. the tree currently has no flowers and is situated meters from a creek. it is quite a large mature tree and is definitely a floribunda
No idea what will come of this extraction but keen to get an idea of the alkaloid activity in this tree. i have done 3 extractions on 3 different floribundas with no success, but have never tried rootbark. I have previously done 2 extractions on phyllodes, both from separate flowering trees, and then later 100g outer layer bark from a mature floribunda which was not in the flowering stage

added 30g caustic soda (lye) to 300ml water

stirred and then slowly added the ground rootbark

just stirred in 200ml warm naptha and soon to add another 100ml

i am aware that i should do 3 separate pulls however i dont have the time at the moment so hopefulyl i can get an idea of what is in this bark with a fairly quick extraction

il keep yas posted!

 

[nen888]

..STB may cause problem with acacia extracts..
ethanol/methanol the most efficient for taking tryptamines out of plant material..
..have heard first-hand of at least 12 (more like 15) successful floribunda stem bark/phyllode extractions..
at a recent lecture was said by a very experienced researcher to be reliable/consistent..

without flower/pod and observation of phyllode glands/veins one cannot be certain of an acacia ID..do not believe location records/councils or even sometimes botanic gardens...

some brands of napatha/shellite almost useless in their holding capacity of alkaloids (i.e don't pull much with even large amounts)

if a single tree is non-yielding, try another

this is the currently the most 'happening' species in Victoria from word of mouth

if you are in any other state (except NSW) it very well may not be floribunda..

ps. 30 grams is not much, cool..but larger amounts would lead to death of branches and eventually sometimes whole tree..

 

[acacian]

hey mate thanks for the info there, it really encouraging to hear successful reports of foribundas as they are one of the most common acacias in my area. i am pretty certain the species i used is a floribunda, as i am well familiar with the appearance of the tree... maybe il upload some pics just to confirm though

is the stb a problem because of the plant fats? i was thinking even if this was the case, that i could just dissolve the fats at the end and recrystalise.. i didnt want to do an acid base extraction as i was only using 30mgs of bark and didnt think it was worth the longer extraction

do you know what kind of yields people have been getting off these trees?

i live in victoria by the way, and i can see how that is the case considering how many of them there are

 

[acacian]

jut collected a bag of phyllodes too... how many grams would be good for an extraction with a decent yield?

 

[nen888]

..friends report 0.3-0.4% alkaloids from phyllodes, 0.5-0.8% bark of floribunda..so 10 grams phyllodes theoretically yields 30-40mg..i don't know the seasonal variance of this species..
re: A/B test extractions..the acuminata extract reported in acacia thread was done in about 4 hours..3 x 40 min boil in ethanol/water/vinegar..basify..NP solvent extract..fan heater evaporate..powdered or ground material is preferable but not essential..

 

[acacian]

what would be the best solvent to use for deffatting the phyllodes? i hear they contain a lot of oils and plant gunk

 

[nen888]

..most common ones ok (eg. napatha), though the 'oils' in phyllodes have usually been found to be alkaloids, which may be desirable..i have never observed that many advantages in defatting..salty basic water washing of 'colour' (other plant components) from NP solvent is helpful..

 

[dromedary]

I've used various turpentine substitutes for acacia leaf extracts in the past. I find that it takes at least two acid washes to get rid of all the plant gunk, oils and fats, but aside from that little inconvenience the usual A/B steps and ingredients work well enough for me.