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Alkaloids in juvenile roots

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Hieronymous

Rising Star
Does anyone know of any analysis of juvenile roots of M. tenuiflora ?

Recently after reading a thread someone posted here I decided to transfer some Jurema seedlings into a passive hydroponic system. It is a very primitive setup in normal pots filled with expanded clay sitting in small plastic tubs for a reservoir. I've been feeding them a solution that my worm buddies brew up for me.

After about a week they started to show roots coming out of the bottom of the pots and look like they will produce a considerable amount of easily harvested biomass. Obviously the biomass will have a very high water content and won't weigh much when dried, but it can be trimmed very easily and replaces itself with new growth every few days. I have trimmed some and didn't seem to affect the plant at all.

From what I've observed Jurema grown this way is very tolerant of "wet feet" that is the roots coming out of the pots are completely submerged. The plants look very happy in this setup considering the recent drop in temperature around here. Other plants from the same batch of seed growing in soil have started dropping foliage where the hydro ones haven't.

I did a web search on alkaloids in juvenile roots and found a paper (that I can't access) that seems to indicate that alkaloids are present in high concentrations in the juvenile roots of Papaver somniferum but nothing specific to Mimosaceae.

If there's magic in the roots at that early stage then this could be adapted for a system with a perpetual harvest.
 
:thumb_up: 8) :thumb_up:

While I (so far) have not seen an analysis of Mimosa seedling roots, or anything 100% relevant ... The DEA, in a paper I'm attaching claims DMT is present even within the seeds of Mimosa hostilis (and P. viridis).
The DEA said:
DMT is present in many plants and their seeds, including in Mimosa hostilis and Psychotria viridis [1-3], and can be abused by smoking, injection, or ingestion of either these natural materials or their crude or purified extracts, either alone or in combination with other extracts (e.g., Ayahuasca [4].)
Somewhat possibly relevant (though not Mimosa)...
DMT production in Phalaris aquatica seedlings said:
The concentration of intermediates increased rapidly from zero in the seeds to maximal values of 25 and 53 micromolar at day 5 for tryptamine (T) and N-methyltryptamine (MT), respectively, 1000 micromolar at day 6 for TRP, and 650 micromolar at day 8 for DMT. The concentration of DMT and of all the intermediates in its synthesis declined rapidly after the maximal value had been reached.
I recommend a small scale extraction to find out (please let us know the results if that occurs).

If anything changes, I'll edit this post.
 

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Thanks for the feedback Cosmic!

It sounds like the idea with Mimosa may have some potential. When I get enough root material I'll do some invetigation

That's interesting about Phalaris seeds, maybe we should be growing them like alfalfa sprouts en masse. An 8 day turnover would be awesome.
 
I would advocate experiments with Deep Water Culture. I will be out of the country for several months this summer, but I'm planning on playing with M. tenuiflora and T. iboga in DWC setups when I get back. I have some other plants I'd also like to try this with as well, but I can't see this happening, for me, until October.
 
That's the plan Snozz, after seeing the way the roots thrive in water it has be tested out.

I'm working on a DWC setup now.

I thought about Aeroponics, but it's not fail safe without backup power supplies etc. DWC is much simpler and I like to keep things as simple as possible.
 
I germed 10 juremas 5 days ago via hot water soak. The smaller ones seem non-viable but the larger ones opened the next day. (I assume they were either immature or the hot water destroyed them?)
CAM00432_1.jpg


This was taken tonight. Five strong seedlings. Tomorrow will be the first day of full sun under the dome. These things are hardy.
CAM00456_1.jpg


I too am interested in the alkaloid content of younger plants. This may be abstract but my idea is to grow these outdoors in potted plots. I want to emulate the perpetual harvest techniques used by cannabis growers and keep a constant source of live plants by cloning. I envision a two year system with yearly harvests after the second year. Starting with mothers or seeds for 50 seedlings to start the cycle. The first year would include raising the plants in pots, transplanting as needed with heavy nutes. At the end of this cycle 50 new seedlings would be started. The second year would have two plots, The plants from last year and the seedlings. At the end of this cycle the elder plants would be harvested and new seedlings would again be started. At this point it will be perpetual with yearly harvests.
There are two main contributors to this idea. My geographical location would allow me to get very impressive results but I rent and simply cant grow naturally. Also, The isolation of alkaloids and research in Phalaris has led me to believe this could be viable at this scale.
 
Thanks for your thoughts, if it works you will have a quick way to generate root mass without having to grow out trees to maturity.

Let us know how it pans out.

I was going to add a few pics of what I've done but my phone has died and I can't find my camera.
 
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