SWIM tested fraction 3 for activity at 7 mg vaporized. It immediately produced very slight nausea for about 30 seconds and tingling sensations in the head. The slight nausea was completely gone in 1 minute. The tingling started to expand to the rest of the body becoming very pleasant, as expected. Visual effects started in about 2 minutes and peaked after about 15 minutes. The entire experience lasted about 90 minutes. Up until the peak there were lots of auditory hallucinations. From the peak onwards the experience was extremely euphoric. The very mild side effects lasted less than 1 minute while the rest of the experience was completely side effect free.

Fraction 3 was obviously pretty clean bufotenine.

Unfortunately, separation using flash chromatography didn’t get rid of the amber coloring. All fractions had the amber coloring to various degrees with fraction 4 being the darkest amber.

The amber material eluted best in fraction 4 which was 25 % ethyl acetate and 75 % methanol.

Does anyone have any idea what this amber coloring is?

Any idea how to remove it from fraction 3? He tried activated carbon but it ate up all the bufotenine.