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SWIM used a very tiny column for this test. It was a standard medicine dropper about 3 inches long and about 1/4 of an inch thick. He plugged it with cotton and added 2 inches of flash chromatography silica that was topped with sand. He used vacuum instead of pressure (its much easier) to elute the solvent mixes. The amount of extract he used was 100 mg dissolved in 0.5 ml of ethyl acetate and 0.5 ml of methanol. The column used was really smaller than it should be fur that amount. But it worked.

The solvent volumes were 4 ml for each fraction.

So a very cheap way to purify bufotenine from an Anadenathera alkaloid mix is to use flash chromatography, and elute the DMT N-Oxide (or whatever it actually is, I’m not sure) with ethyl acetate, and then the bufotenine with a 3:1 mix with ethyl acetate and methanol.

By doing this simple procedure, you can get rid of the sticky texture of an Anadenathera alkaloid extract and get rid of nearly all the side effects.

Don’t be put off by the flash column chromatography. It’s very easy to perform. Even a child could do it. You can use a standard medicine dropper as your column. You don’t need fancy equipment. You will need a source of vacuum or pressure to speed up the process. You will also need flash chromatography silica gel, a little sand, the solvents, some cotton, and any kind of column (a medicine dropper is great for small quantities).

Pure bufotenine is far nicer than the alkaloid mix you usually get by extracting from Anadenathera using an A/B tech. The side effects experience from the alkaloid mix can be pretty intense. The batch SWIM used for this test was extremely weak and produced lots of side effects (body tension, head tension, long lasting nausea, and other unpleasant bodily sensations).

If you’re interested at all in extremely pure bufotenine, this is the way to go. SWIM thinks bufotenine (5-HO-DMT) is far better than DMT as a vaporized experience, but orally, DMT is better. Vaporized bufotenine lasts about 90-120 minutes (depending on dosage), it causes almost no “mind fuck” at all, and it causes both visual and auditory hallucinations. It has very mild side effects which only last about 30 seconds (very slight nausea and tingling in the head).

<blockquote data-quote="69ron" data-source="post: 3231979" data-attributes="member: 787">SWIM used a very tiny column for this test. It was a standard medicine dropper about 3 inches long and about 1/4 of an inch thick. He plugged it with cotton and added 2 inches of flash chromatography silica that was topped with sand. He used vacuum instead of pressure (its much easier) to elute the solvent mixes. The amount of extract he used was 100 mg dissolved in 0.5 ml of ethyl acetate and 0.5 ml of methanol. The column used was really smaller than it should be fur that amount. But it worked.The solvent volumes were 4 ml for each fraction.So a very cheap way to purify bufotenine from an Anadenathera alkaloid mix is to use flash chromatography, and elute the DMT N-Oxide (or whatever it actually is, I’m not sure) with ethyl acetate, and then the bufotenine with a 3:1 mix with ethyl acetate and methanol.By doing this simple procedure, you can get rid of the sticky texture of an Anadenathera alkaloid extract and get rid of nearly all the side effects.Don’t be put off by the flash column chromatography. It’s very easy to perform. Even a child could do it. You can use a standard medicine dropper as your column. You don’t need fancy equipment. You will need a source of vacuum or pressure to speed up the process. You will also need flash chromatography silica gel, a little sand, the solvents, some cotton, and any kind of column (a medicine dropper is great for small quantities).Pure bufotenine is far nicer than the alkaloid mix you usually get by extracting from Anadenathera using an A/B tech. The side effects experience from the alkaloid mix can be pretty intense. The batch SWIM used for this test was extremely weak and produced lots of side effects (body tension, head tension, long lasting nausea, and other unpleasant bodily sensations).If you’re interested at all in extremely pure bufotenine, this is the way to go. SWIM thinks bufotenine (5-HO-DMT) is far better than DMT as a vaporized experience, but orally, DMT is better. Vaporized bufotenine lasts about 90-120 minutes (depending on dosage), it causes almost no “mind fuck” at all, and it causes both visual and auditory hallucinations. It has very mild side effects which only last about 30 seconds (very slight nausea and tingling in the head).</blockquote>

[QUOTE="69ron, post: 3231979, member: 787"] SWIM used a very tiny column for this test. It was a standard medicine dropper about 3 inches long and about 1/4 of an inch thick. He plugged it with cotton and added 2 inches of flash chromatography silica that was topped with sand. He used vacuum instead of pressure (its much easier) to elute the solvent mixes. The amount of extract he used was 100 mg dissolved in 0.5 ml of ethyl acetate and 0.5 ml of methanol. The column used was really smaller than it should be fur that amount. But it worked. The solvent volumes were 4 ml for each fraction. So a very cheap way to purify bufotenine from an Anadenathera alkaloid mix is to use flash chromatography, and elute the DMT N-Oxide (or whatever it actually is, I’m not sure) with ethyl acetate, and then the bufotenine with a 3:1 mix with ethyl acetate and methanol. By doing this simple procedure, you can get rid of the sticky texture of an Anadenathera alkaloid extract and get rid of nearly all the side effects. Don’t be put off by the flash column chromatography. It’s very easy to perform. Even a child could do it. You can use a standard medicine dropper as your column. You don’t need fancy equipment. You will need a source of vacuum or pressure to speed up the process. You will also need flash chromatography silica gel, a little sand, the solvents, some cotton, and any kind of column (a medicine dropper is great for small quantities). Pure bufotenine is far nicer than the alkaloid mix you usually get by extracting from Anadenathera using an A/B tech. The side effects experience from the alkaloid mix can be pretty intense. The batch SWIM used for this test was extremely weak and produced lots of side effects (body tension, head tension, long lasting nausea, and other unpleasant bodily sensations). If you’re interested at all in extremely pure bufotenine, this is the way to go. SWIM thinks bufotenine (5-HO-DMT) is far better than DMT as a vaporized experience, but orally, DMT is better. Vaporized bufotenine lasts about 90-120 minutes (depending on dosage), it causes almost no “mind fuck” at all, and it causes both visual and auditory hallucinations. It has very mild side effects which only last about 30 seconds (very slight nausea and tingling in the head). [/QUOTE]