The current hypothesis going around on the nexus concerning the side effects of bufotenin are that it is caused by bufotenin n oxide.
SWIM has performed both GC-MS and LC-MS analysis of bufotenin purified using the Ott 2001 method in the journal of psychoactive drugs (SWIM thinks thats the source). The method was taken from an earlier work on isolation of bufotenin. The only compound present is bufotenin and a trace amount of dmt. No n oxide at all.
SWIM at first thought that perhaps the n oxide might have degraded in the injection of the GC-MS (set at 280 degrees C). But SWIM was always puzzled by why wouldn't that also happen when you smoke bufotenin? So SWIM decided to do LC-MS analysis using AP-ESI as the ionization mode. This is a softer ionization MS procedure then EI which was used in GC-MS which should increase the intensity of the molecular ion peak. It did. But the molecular ion peak was 205 bufotenin +1 hydrogen. Exactly as expected. Furthermore SWIM doesn't think that the n oxide is degrading in the MS giving a false result for bufotenin because the n oxide would seperate differently in the HPLC column then the parent compound. Its easy to seperate n oxides from their parent compounds by LC.
Therefore SWIM thinks this talk of n oxide bufotenin should be laid to rest. SWIM thinks the reason people experience side effects from bufotenin and preparations there of is because of the bufotenin itself and possibly other impurities in the seeds. SWIMs material however is very pure and SWIM always gets side effects. The only time the side effects go away is if SWIM keeps smoking the material and gets a kind of tolerance to that effect.
SWIM can provide the data if people are not convinced. But unless you know a lot about mass spectrometry you won't be able to interpret it.
SWIM has performed both GC-MS and LC-MS analysis of bufotenin purified using the Ott 2001 method in the journal of psychoactive drugs (SWIM thinks thats the source). The method was taken from an earlier work on isolation of bufotenin. The only compound present is bufotenin and a trace amount of dmt. No n oxide at all.
SWIM at first thought that perhaps the n oxide might have degraded in the injection of the GC-MS (set at 280 degrees C). But SWIM was always puzzled by why wouldn't that also happen when you smoke bufotenin? So SWIM decided to do LC-MS analysis using AP-ESI as the ionization mode. This is a softer ionization MS procedure then EI which was used in GC-MS which should increase the intensity of the molecular ion peak. It did. But the molecular ion peak was 205 bufotenin +1 hydrogen. Exactly as expected. Furthermore SWIM doesn't think that the n oxide is degrading in the MS giving a false result for bufotenin because the n oxide would seperate differently in the HPLC column then the parent compound. Its easy to seperate n oxides from their parent compounds by LC.
Therefore SWIM thinks this talk of n oxide bufotenin should be laid to rest. SWIM thinks the reason people experience side effects from bufotenin and preparations there of is because of the bufotenin itself and possibly other impurities in the seeds. SWIMs material however is very pure and SWIM always gets side effects. The only time the side effects go away is if SWIM keeps smoking the material and gets a kind of tolerance to that effect.
SWIM can provide the data if people are not convinced. But unless you know a lot about mass spectrometry you won't be able to interpret it.