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Chemical Composition of Traditional and Analog Ayahuasca

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Jagube

Jagube

Esteemed member
I can't access the paper, but apparently it has some interesting info, from what I've gathered from this Spanish article: Un estudio científico desvela la composición química de cien muestras de ayahuasca - Plantaforma para la Defensa de la Ayahuasca

Original paper: https://www.tandfonline.com/doi/abs/10.1080/02791072.2020.1815911

The study aimed to determine concentrations of the main bio- and psychoactive components of ayahuasca used in different locations and traditions. We collected 102 samples of brews from ayahuasca-using communities. Concentrations of N,N-dimethyltryptamine (DMT), tetrahydroharmine, harmine, and harmaline were determined by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Qualitative analyses for non-traditional additives (moclobemide, psilocin, yuremamine) were performed by high resolution mass spectrometry. Higher and more variable concentrations of DMT in neoshamanic ayahuasca samples compared to indigenous samples may indicate use of higher and more variable proportions of DMT-containing admixture plants. From European samples, we found two related samples of analog ayahuasca containing moclobemide, psilocin, DMT, yuremamine, and very low concentrations of B. caapi alkaloids. Some analogs of ayahuasca (Peganum harmala, Mimosa tenuiflora) were used in Europe. No analogs were found from Brazil or Santo Daime ceremonies in Europe.
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Thanks ShamensStamen. This is interesting:

Decline of THH/HME ratio
with the degree of Daime corresponds to longer boiling
times and also to increasing number of repeated
extractions of the same plant material during feitio,
and may be related to different qualitative subjective
effects of different degrees of Daime
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It doesn't quite fit the hypothesis that harmaline gets reduced to THH in the decoction process...?

Average (minimal – maximal)
percentages from the sum of the concentrations of the
4 analytes were: DMT 26.2% (9.2–63.7%); THH 29.8%
(1.4–55.4%); harmine 41.5% (15.0–75.0%); harmaline
2.5% (0.3–16.8%). [..]

Harmaline/harmine concentration ratios around
0.1 have been detected in brews from Santo Daime
where P. harmala is not used. [..]

As seen in Figure 3, there was also
a trend toward higher DMT/HME ratios among Daime
samples (n = 53) compared to shamanic samples.
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ShamensStamen said:
[...]

Keep that sci-hub.se site in mind, a lot of full papers can be accessed through that site.
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If any particular sci-hub portal doesn't work (e.g., if it's blocked in your area or, especially, if using a VPN hasn't solved the access issue), try connecting through "sci-hub.shop", which redirects to your current viable portal.
 
Interesting find Jagube & thanks for link to paper ShamensStamen. It is the Santo Daime vegetal group that consistently makes brews with next to zero levels of harmaline, saw this in a study. Perhaps we should study how they brew, and if they use vitamin c? and for how long? Vitamin c is after all just like Dr. Shulgin said "an efficient hydrogen donor."
 
A little more from the study: You should know if your harmaline gets converted to tetrahyroharmine, as when mixed with water and smeared on a paper plate, it will glow blue (THH) instead of green (harmaline).

In TIHKAL, 300mg of tetrahydroharmine (THH) is equated by one psychonaut to the closed eye visionary (CEV) power of 100mg harmaline, but without all the nausea and dizziness. It glows blue when mixed with water under blacklight, like LSD or psilocin & has a metallic-like lingering taste with a 10.5 hour half-life.
 

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If we could figure out if/how brewing methods have an impact on this, I would be thrilled to make a big section about it in the Aya sticky (I'm in the middle of reworking it).

Is it possible that the differences are simply a variation in alkaloids between types of vine?

I would be all in to brew/bioassay/take pretty pictures under blacklight for the team. :thumb_up:

Adding vitamin C to a brew can get disgusting quickly, so I'd be curious to hear if anyone knows anything about minimum effective amounts?
 
ms_manic_minxx said:
If we could figure out if/how brewing methods have an impact on this, I would be thrilled to make a big section about it in the Aya sticky (I'm in the middle of reworking it).

Is it possible that the differences are simply a variation in alkaloids between types of vine?

I would be all in to brew/bioassay/take pretty pictures under blacklight for the team. :thumb_up:

Adding vitamin C to a brew can get disgusting quickly, so I'd be curious to hear if anyone knows anything about minimum effective amounts?
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Great point ms_manic_minxx.:) Professor8 here at this forum back in 2010 made THH from harmaline using vitamin C, a kind search will pull up his procedure: "Conversion of Harmine->Harmaline->THH by simple boiling." It's possible the Santo Daime boil for 12 hours or so, but I'm not certain. Conversion of Harmine->Harmaline->THH by simple boiling (and storage?) - DMT Discussion - Welcome to the DMT-Nexus

professor8 (found here from 11/1/2010 he writes like a poet w/special powers of imagination & expression):
Tetrahydroharmine (THH) has the ability to raise your vibration in a most powerful, yet subtle way. It brings a crystalline prismy texture to spice and adds a super clear watery dimension to Aya, like looking down through 10meters of shimmering Caribbean Sea on clear blue day. It brings a dimension of pure light to the entheogenic experience and encourages entities & intelligences of only the Highest Order. If one is not accustomed to perceiving these experiences with a spiritual perspective most of the nuances & subtleties THH brings on are overlooked and remain unseen and one would better enjoy Harmaline as a house painter chooses a roller over a brush, its about preference & choice.
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I agree with his statement. Should add that music sounds quite incredible on a combo of 150mg or more of THH + 60mg sublingual "under the tongue" HPBCD DMT held under tongue for 15 minutes as tetrahydroharmine imho breaks down the filters or barriers in the mind, so that "mind at large" can be let loose, very similar to listening to music on cactus. Strong effects at 10 minutes after sublingual application, 60 to 90 minute duration, tryptamine rush, dilated pupils, greatly elevated heart rate and pulse, CEV visuals in neon colors in combo with 250mg THH taken 3 hours earlier. Open eyed profound beauty, very transcendent: Journal: 50 Sublingual | <span style="frontcolor:pink !Important ;">High Pobability of Braindamage by Creepy non tested Drugs (forced by scammer 69ron)</span> | DMT Ayahuasca journeys over a years time - Pharmahuasca - Welcome to the DMT-Nexus

Mind at Large - Wikipedia

en.wikipedia.org en.wikipedia.org
 
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