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Basing temp doesn't matter if the bark is discarded after multiple acid cooks. But when keeping the bark in same solution Cyb style (one acid cook), the acid doesn't take all actives out which continue to to come out during the basing stage. Basing at higher temp means more actives come out into the solution. 



This was just a test to see what happens with room temp for the entire procedure. I could have also chosen not to freeze/thaw this batch for less variables.



Yes always use higher temp for the acid cook.



Yes for completeness an extra test would be needed: hot pulls on cold acid/base. But you shouldn't acid/base cold anyway.



The percentage is the amount of DMT obtained from extracting vs the total amount of DMT present in the bark? Meaning a 60 min acid cook at 80 C on 100g bark containing 1% DMT yields 0.77g? How did you perform this experiment?


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