Infundibulum, I know it was destined to fail, and in fact it did. I got all too excited and rushed to do some kind of experimentation. Now it's different, I have studied this topic from books and papers, and gathered well-studied protocols.

You are saying this because this thread is more than a month old.

Sucrose is an absolute requirement in the medium since the small plant needs a carbon source but can't yet perform photosynthesis at enough rate. Sucrose is used at a high concentration of 2% w/v. If you read the description of the Murashige and Skoog medium (one of the most used growing mediums), you'll see it listed there.

Callus culture is interesting as it is a good source of unspecialized cells (their growing medium is hormone-free) ready to grow in presence of hormones, but it is also even harder to maintain. It is used at the large and industrial scale. My first attempts will be with an explant.

The primary goal is to learn. I consider this a good project involving biology and chemistry. Experimenting with plants and secondary metabolite production should also be fun.

Thank you for offering your help; I'll let you know when I need it.