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Here's a late update from the butyl acetate variation: some tiny, needle like crystals were obtained from the second attempt, which used completely rotten, dried out and partially-eaten-by-beetle-larvae material. This to me indicates that if assured potent material were to be used, the "CIBLO" variation would work. I think the tiny amount of crystals reflects the tiny amount of alkaloid that was left in the remnants of less decomposed plant material. A lot of the bulk will have comprised waxy outer skin and woody veins, neither of which is going to be particularly rich in alkaloids.It remains to be seen whether the crystalline material recovered after freezing the filtered extract from the first attempt is anything other than citric acid. That attempt was with rotten to the point of complete liquefaction PC pachanoi - another assured way of achieving inconclusive results, it seems Nonetheless, these crystals still need to be weighed and perhaps a recrystallisation from aqueous acetone might be instructive, largely to see if needle-like crystals form instead of the not-quite-right citric acid-like stuff that appeared at first.
Here's a late update from the butyl acetate variation: some tiny, needle like crystals were obtained from the second attempt, which used completely rotten, dried out and partially-eaten-by-beetle-larvae material. This to me indicates that if assured potent material were to be used, the "CIBLO" variation would work. I think the tiny amount of crystals reflects the tiny amount of alkaloid that was left in the remnants of less decomposed plant material. A lot of the bulk will have comprised waxy outer skin and woody veins, neither of which is going to be particularly rich in alkaloids.
It remains to be seen whether the crystalline material recovered after freezing the filtered extract from the first attempt is anything other than citric acid. That attempt was with rotten to the point of complete liquefaction PC pachanoi - another assured way of achieving inconclusive results, it seems Nonetheless, these crystals still need to be weighed and perhaps a recrystallisation from aqueous acetone might be instructive, largely to see if needle-like crystals form instead of the not-quite-right citric acid-like stuff that appeared at first.