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failed at cultivate p.cubensis ?
- Thread starter B1ack_Ph03niX
- Start date
That does look bad 
Please explain exactly how you cultivated it so we can help.. What grain was the spawn grown on? What was the source of the spawn, spore syringe, LC syringe, spore print, agar? What substrate did you grow it on? How did you prepare the substrate, how often did you mist it, how much air exchange did it have, etc etc ?
Please explain exactly how you cultivated it so we can help.. What grain was the spawn grown on? What was the source of the spawn, spore syringe, LC syringe, spore print, agar? What substrate did you grow it on? How did you prepare the substrate, how often did you mist it, how much air exchange did it have, etc etc ?
downwardsfromzero
Boundary condition
That looks like an entirely different species of mushroom altogether! Maybe Pleurotus, Grifola or Sparassis - but even then I'd say there are 'multiple issues'.
Yeah, one of them seems like pink oyster... quite amazing really lol
That looks like some other mold took hold before the spores did. Real fuzzy looking.
B1ack_Ph03niX
Rising Star
endlessness said:That does look bad
Please explain exactly how you cultivated it so we can help.. What grain was the spawn grown on? What was the source of the spawn, spore syringe, LC syringe, spore print, agar? What substrate did you grow it on? How did you prepare the substrate, how often did you mist it, how much air exchange did it have, etc etc ?
What was the source of the spawn, spore syringe, LC syringe, spore print, agar? i spore printed it by myself mounts ago (Sterile tinfoil and printed on it)and kept it in
refrigerator mounts ago.
What grain was the spawn grown on? i made the cakes in corn grain
What substrate did you grow it on? after the cakes readied i kept them under cold water for 24 hours then rolled them in vermiculite and placed them in Tupperware .
how often did you mist it, how much air exchange did it have, etc etc ? i didn't mist it since it misted by perlit i placed under it, every day i opened then Tupperware for air exchange.
my last successful flushes that i spore printed them, after the cakes had been prepared i put them in yard and they fruited successfully, i gather them from my yard. maybe when i gathered them they get some mold and ruined my spoor print.
i hope i answered all you question
thanks for caring
So you spore printed it and went straight to grain.. I think that might be your issue. While your foil might be sterile, the mushroom itself isn't, so the mushroom may contain spores from other fungus around. Sometimes people get the sporeprint working fine but from what I've been reading, the experienced growers generally go from sporeprint to agar, to separate the cubensis mycelium from other contamination, and only after agar they innoculate the grain.
So you can either research into agar if you havent yet and try to clean your sporeprints, or get yourself an LC syringe that was previously clean with agar, or some spawn to start with, from some supplier.
As for air exchange, maybe more than once a day venting is prob better but either way I think your main issue was probably the contamination from the sporeprint
Good luck with your future grows!
So you can either research into agar if you havent yet and try to clean your sporeprints, or get yourself an LC syringe that was previously clean with agar, or some spawn to start with, from some supplier.
As for air exchange, maybe more than once a day venting is prob better but either way I think your main issue was probably the contamination from the sporeprint
Good luck with your future grows!