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Again thx for reading and thinking ! And again, this topic is THEORETICAL. The manufacturing of DMT (including extraction and synthesis) is illegal in most parts of the world.

[USER=3685]@Xt[/USER] & [USER=535]@endlessness[/USER]: As mentioned, im just a spirit, so still figuring out my way of exploring the Nexus. My post is certainly NOT suggesting ANY process, which ANY person can do at home. Whithout lots of expensive materials and analytical means (and of course know-how) it is not accomplishable. Furthermore I would never have the idea to administer any of theoretical obtained product to any living thing which includes myself. [Uh, not to mention my studies included GxP in the pharmaceutical industrie.]

I conisider it as a shame that studies on psychedelic substances are an area, which all governments forbid. I am sure, that the military does lots of "top secret" stuff, and they obtained lots of results... but they WON'T share with the public. I see the nexus as a great oppurtunity to share, evaluate and explore the world of psychedelics: it is wrong to forbid them. Education is the key. Nexus is the key. It dosn't matter who you are, a pothead (sorry potheads, i mean it in a positive way) or a crazy scientist - we all want to expand our horizons.

[USER=6470]@Newfound_wonder[/USER]: Theoretically yes. Just get a kg of l-tryptophane, let the first enzyme work, inactivate the first enzyme and let the second do its job. Thats the simple theory, but real life looks different - enzymes need different conditions (including pH, temp....), cofactors etc... furthermore the purification of enzymes in larger quantities is quite tricky. Thats why the theoretical strategy involves in-vivo first.

@Infundubulum: Yeah proof of principle would be great for beginning Anyways there is no way of spending MY money on this :lol: The effort... well, people put a lot of effort in different kind of (crazy) things, consider it as a hobby of mine . The overall achivement - well considering the tiny tiny quantities of DMT present in natural sources and a long way of chemical synthesis - there should be an alternative way to acess great quantities of DMT, which would be neccessary for lets say reasearch - if one day somehow it should be legalized...
Coming to the smell. Well, I worked with crazy ass bacterial and fungal cultures, THEY REALLY SMELLED LIKE SHIT. Thus I consider colis and yeasts as relative mild. Thinking about it, you are maybe right, havent worked since a long time with yeast, so the actually might smell better. BUT considering smell: when I read, that people extract their DMT with naphtha - geeeeee - my hair stands up like an urchin. I would't even lighten my BBQ with that nasty smelling crap...

[USER=6370]@joedirt[/USER]: As you mentioned aromatic amino acid decarboxylase is present in most organisms... yeah. But the promoter is not strong enough for large scale. AADC is one son of a ...
INMT: easy, done it in a fictional world. In this fictional and theoretical world, the results from expressing the genes and extracting, GS-MS would show quantities of about 5µg nnDMT / mL of bacterial culture. But as mentioned before, experiments need to be repeated. Sometimes I mess up stuff in my fictional world....

[USER=1900]@SWIMfriend[/USER]: Ahhh, nice. Someone wise Yepp, there would be a method for (almost)fast screening. My fastest way is by thin layer chromatography. Obtaining positive results from TLC would bring us to GC-MS. Run takes about 30 minutes (not counting the wash cycles and heating period). Outright synthesis and plant extracts are quite well known, I am looking for a NEW theoretical way. Spending sometimes 70h a week in a lab gives you crazy ideas, you know, all the waiting time in the lab would be a waste of time.

<blockquote data-quote="crystalizer" data-source="post: 3444608" data-attributes="member: 9193">Again thx for reading and thinking ! And again, this topic is THEORETICAL. The manufacturing of DMT (including extraction and synthesis) is illegal in most parts of the world. [USER=3685]@Xt[/USER] &amp; [USER=535]@endlessness[/USER]: As mentioned, im just a spirit, so still figuring out my way of exploring the Nexus. My post is certainly NOT suggesting ANY process, which ANY person can do at home. Whithout lots of expensive materials and analytical means (and of course know-how) it is not accomplishable. Furthermore I would never have the idea to administer any of theoretical obtained product to any living thing which includes myself. [Uh, not to mention my studies included GxP in the pharmaceutical industrie.]I conisider it as a shame that studies on psychedelic substances are an area, which all governments forbid. I am sure, that the military does lots of &quot;top secret&quot; stuff, and they obtained lots of results... but they WON&#039;T share with the public. I see the nexus as a great oppurtunity to share, evaluate and explore the world of psychedelics: it is wrong to forbid them. Education is the key. Nexus is the key. It dosn&#039;t matter who you are, a pothead (sorry potheads, i mean it in a positive way) or a crazy scientist - we all want to expand our horizons. [USER=6470]@Newfound_wonder[/USER]: Theoretically yes. Just get a kg of l-tryptophane, let the first enzyme work, inactivate the first enzyme and let the second do its job. Thats the simple theory, but real life looks different - enzymes need different conditions (including pH, temp....), cofactors etc... furthermore the purification of enzymes in larger quantities is quite tricky. Thats why the theoretical strategy involves in-vivo first. @Infundubulum: Yeah proof of principle would be great for beginning <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite2" alt=";)" title="Wink&nbsp; &nbsp; ;)" loading="lazy" data-shortname=";)" /> Anyways there is no way of spending MY money on this :lol: The effort... well, people put a lot of effort in different kind of (crazy) things, consider it as a hobby of mine <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite1" alt=":)" title="Smile&nbsp; &nbsp; :)" loading="lazy" data-shortname=":)" />. The overall achivement - well considering the tiny tiny quantities of DMT present in natural sources and a long way of chemical synthesis - there should be an alternative way to acess great quantities of DMT, which would be neccessary for lets say reasearch -&nbsp; if one day somehow it should be legalized...&nbsp; Coming to the smell. Well, I worked with crazy ass bacterial and fungal cultures, THEY REALLY SMELLED LIKE SHIT. Thus I consider colis and yeasts as relative mild. Thinking about it, you are maybe right, havent worked since a long time with yeast, so the actually might smell better. BUT considering smell: when I read, that people extract their DMT with naphtha - geeeeee - my hair stands up like an urchin. I would&#039;t even lighten my BBQ with that nasty smelling crap... [USER=6370]@joedirt[/USER]: As you mentioned aromatic amino acid decarboxylase is present in most organisms... yeah. But the promoter is not strong enough for large scale. AADC is one son of a ... INMT: easy, done it in a fictional world. In this fictional and theoretical world, the results from expressing the genes and extracting, GS-MS would show quantities of about 5µg nnDMT / mL of bacterial culture. But as mentioned before, experiments need to be repeated. Sometimes I mess up stuff in my fictional world.... [USER=1900]@SWIMfriend[/USER]: Ahhh, nice. Someone wise <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite1" alt=":)" title="Smile&nbsp; &nbsp; :)" loading="lazy" data-shortname=":)" /> Yepp, there would be a method for (almost)fast screening. My fastest way is by thin layer chromatography. Obtaining positive results from TLC would bring us to GC-MS. Run takes about 30 minutes (not counting the wash cycles and heating period). Outright synthesis and plant extracts are quite well known, I am looking for a NEW theoretical way. Spending sometimes 70h a week in a lab gives you crazy ideas, you know, all the waiting time in the lab would be a waste of time.</blockquote>

[QUOTE="crystalizer, post: 3444608, member: 9193"] Again thx for reading and thinking ! And again, this topic is THEORETICAL. The manufacturing of DMT (including extraction and synthesis) is illegal in most parts of the world. [b][USER=3685]@Xt[/USER] & [USER=535]@endlessness[/USER]:[/b] As mentioned, im just a spirit, so still figuring out my way of exploring the Nexus. My post is certainly NOT suggesting ANY process, which ANY person can do at home. Whithout lots of expensive materials and analytical means (and of course know-how) it is not accomplishable. Furthermore I would never have the idea to administer any of theoretical obtained product to any living thing which includes myself. [Uh, not to mention my studies included GxP in the pharmaceutical industrie.] I conisider it as a shame that studies on psychedelic substances are an area, which all governments forbid. I am sure, that the military does lots of "top secret" stuff, and they obtained lots of results... but they WON'T share with the public. I see the nexus as a great oppurtunity to share, evaluate and explore the world of psychedelics: it is wrong to forbid them. Education is the key. Nexus is the key. It dosn't matter who you are, a pothead (sorry potheads, i mean it in a positive way) or a crazy scientist - we all want to expand our horizons. [b][USER=6470]@Newfound_wonder[/USER]:[/b] Theoretically yes. Just get a kg of l-tryptophane, let the first enzyme work, inactivate the first enzyme and let the second do its job. Thats the simple theory, but real life looks different - enzymes need different conditions (including pH, temp....), cofactors etc... furthermore the purification of enzymes in larger quantities is quite tricky. Thats why the theoretical strategy involves in-vivo first. [b]@Infundubulum:[/b] Yeah proof of principle would be great for beginning ;) Anyways there is no way of spending MY money on this :lol: The effort... well, people put a lot of effort in different kind of (crazy) things, consider it as a hobby of mine :). The overall achivement - well considering the tiny tiny quantities of DMT present in natural sources and a long way of chemical synthesis - there should be an alternative way to acess great quantities of DMT, which would be neccessary for lets say reasearch - if one day somehow it should be legalized... Coming to the smell. Well, I worked with crazy ass bacterial and fungal cultures, THEY REALLY SMELLED LIKE SHIT. Thus I consider colis and yeasts as relative mild. Thinking about it, you are maybe right, havent worked since a long time with yeast, so the actually might smell better. BUT considering smell: when I read, that people extract their DMT with naphtha - geeeeee - my hair stands up like an urchin. I would't even lighten my BBQ with that nasty smelling crap... [b][USER=6370]@joedirt[/USER]:[/b] As you mentioned aromatic amino acid decarboxylase is present in most organisms... yeah. But the promoter is not strong enough for large scale. AADC is one son of a ... INMT: easy, done it in a fictional world. In this fictional and theoretical world, the results from expressing the genes and extracting, GS-MS would show quantities of about 5µg nnDMT / mL of bacterial culture. But as mentioned before, experiments need to be repeated. Sometimes I mess up stuff in my fictional world.... [b][USER=1900]@SWIMfriend[/USER]:[/b] Ahhh, nice. Someone wise :) Yepp, there would be a method for (almost)fast screening. My fastest way is by thin layer chromatography. Obtaining positive results from TLC would bring us to GC-MS. Run takes about 30 minutes (not counting the wash cycles and heating period). Outright synthesis and plant extracts are quite well known, I am looking for a NEW theoretical way. Spending sometimes 70h a week in a lab gives you crazy ideas, you know, all the waiting time in the lab would be a waste of time. [/QUOTE]