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While having a shower, i spotted a minor mistake.

I was deep in joedirt's thoughts about AADC being present in most organisms anyways and thus only the cloning of INMT would ne neccessary.

So the correction would be, of course you clone till point 18. both genes in parallel on its own. [LOL, hope you know what I mean]. Thus two different expression vectors looking at the selection marker are neccessary.

When you have both expression vectors confirmed, you would prepare competent cells of one of your GMOs, either the AADC or the INMT strain and transform them with the other one...

Then you can go on with point 19

AAAAND now, really off to work

<blockquote data-quote="crystalizer" data-source="post: 3444654" data-attributes="member: 9193">While having a shower, i spotted a minor mistake.I was deep in joedirt&#039;s thoughts about AADC being present in most organisms anyways and thus only the cloning of INMT would ne neccessary. So the correction would be, of course you clone till point 18. both genes in parallel on its own. [LOL, hope you know what I mean]. Thus two different expression vectors looking at the selection marker are neccessary. When you have both expression vectors confirmed, you would prepare competent cells of one of your GMOs, either the AADC or the INMT strain and transform them with the other one... Then you can go on with point 19 <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite2" alt=";)" title="Wink&nbsp; &nbsp; ;)" loading="lazy" data-shortname=";)" />AAAAND now, really off to work <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite2" alt=";)" title="Wink&nbsp; &nbsp; ;)" loading="lazy" data-shortname=";)" /></blockquote>

[QUOTE="crystalizer, post: 3444654, member: 9193"] While having a shower, i spotted a minor mistake. I was deep in joedirt's thoughts about AADC being present in most organisms anyways and thus only the cloning of INMT would ne neccessary. So the correction would be, of course you clone till point 18. both genes in parallel on its own. [LOL, hope you know what I mean]. Thus two different expression vectors looking at the selection marker are neccessary. When you have both expression vectors confirmed, you would prepare competent cells of one of your GMOs, either the AADC or the INMT strain and transform them with the other one... Then you can go on with point 19 ;) AAAAND now, really off to work ;) [/QUOTE]