?????

Well, no really need to get in such an exhaustive detail, it is not necessary.  Just design the primers if you got something more meaningful to contribute and outline a strategy. Because I see that you got some ideas but not a plan. You initiate this discussion of transforming cells with DNA to make dmt and you have not even decided what type of DNA you transform them with! Which enzymes? from which organism(s)? why did you choose them? Do you intend to use whole ORF or parts of them?  

You see what I mean? We have a really long way to go in the discussion and planning before throwing a bunch of meaningless technical details. Until now it has been just mental masturbation. Do not get lost in the details especially since you surely know that many of these descriptions and numbers do not matter. I really don't care one bit if your pcr is 20 microliters or 33 or if your o/n cultures are 2 or 5 ml and most likely whether your primers self-anneal or whatever is the least of your concerns. your whole post reads as a show off to be honest!