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Was not sure what you wanted... sry for that, misunderstanding.
course ive planned sth out, i thought that you are going to do some fantasy work at your lab, and how do i know what you got in stock...mental masturbation looks different, belive me.

Of course whole ORF.

1) Mesorhizobium loti MAFF303099 chromosome, complete genome
NCBI Reference Sequence: NC_002678.2
LOCUS    NC_002678    1554 bp DNA    linear   BCT 29-JUN-201

mlr4653, aromatic-L-amino-acid decarboxylase

ORIGIN
1 atgccgagga gatggcgctg tcgcgctcga cgcccggcgg ccagttcccg gcggcttgac
   61 gggagcccgg tcatggacat cgcgcaggaa accgtcggca ccgaggaaac gctcgacccc
121 gccgactggg ccgcagtgca agccctgtcg caccaggtca tcgacgatgc cgtcgactat
181 ctcagcgaca tcagggaccg cccggcctgg cgcgagatgc cggcggaggt gcgagaagcc
241 ttcgcggcac ctctgccgcg atcgccggtg ccgctggccg ccgtctatga cgaggtctcg
301 cgcacggtga tgtcctatcc gatgggcaac atccatccgc gattctgggc ctggtatatg
361 gggtcgagca acttcaccgg cgcgctcggc gacttcctgg cggcaatcca gggttccaac
421 ctcggcggcg gcaaccacgc cgccgggctg atggacagcc aggtggtcaa ctggtgcaag
481 cagatgctgg gctttccggc ttccgcctcc ggcacgctgg tcagcggcgg ctcgatggcc
541 aacgtgatcg gcctgaccgt ggcgcgcaac gccaaggccg gcatcgacgt gcgcgagcac
601 ggcgtcgcgg cgatcgaaaa gccgctgcgc ttctatggct cggaccagat ccattcctgc
661 caccgcaagg caatggaagc acttggcctc ggcaaccggg cgctgcggcg catcccgacg
721 gatgccgacc tgcgcatcga cataccggcc ttgcgcgcgg cgatcgttga ggaccgcgaa
781 gccggtttca agccggcctg cgtcatcggc aatgccggca cggtcaacac cggcgcgatc
841 gacgatttgc gggcgcttgc caaactcgcg catgaggaag gcctatggtt ccatgtcgat
901 ggctgcatcg gcgcgctgat cgcgatcgcc cccgacaatg cgcatcgcgt tgccggcatc
961 gaatgggcgg attccgtcgc gctcgatccg cacaaatggc tgcatgcccc gttcgaggtc
   1021 ggctgcgccc tggtcaggga tgccgtcgcc caccgccgga ccttcgcggt gacgccggaa
   1081 tatctggaat cgacgccgcg cggccttgcc tccggcgaat ggctgcacga ttacggcctg
   1141 cagacgtcgc gcggctttcg cgcgctgaaa gtctggatgg cgctgaagga acatggtgtc
   1201 gacaaattcg gccgcctgat cgaccagaac atcgcgcaag cctcctatct cgccggggtg
   1261 gtcgaggccg agccgctgat ggagctggcg acgtcgccga ccatcaacat cgtctgcttc
   1321 cgctaccagc ccggcgtcac cggcgaggcg ctgaaggtgc tcaacaccga gatcatgctg
   1381 cggctgcagg aacagggcat cgccgtcctt tccgacacta ccgtgcatgg cgaacactgg
   1441 ctgcgcgtgg cgatcgccaa ccaccgcaca aggcgcgagg acctcgacct tctggtcgag
   1501 gaaacggtgc ggttgggacg tgagatcgca gcagaaggct ccccccggaa atag

fw   atGGATCC atgccgagga gatggcgctg
rev atCCCGGG CTATTTCCGGGGGGAGCCTT

reverse primer would suck. not equally distributed. better primer MUST be outside ORF.

2) MO as above, gene="mlr4870", arylamine N-acetyltransferase

ORIGIN
1 atgaacgatg ctcctccctt cgacctcgac gcctatctcg ctcgcatcgg ttacaccggt
   61 ccgcgcaacg cgtcgctgga tacgctgaag gcgctgcatt tcgcgcatcc gcaggcgatc
121 cccttcgaga acatcgatcc gtttcttggt cgcccggtcc ggctcgacct tgccgcgctg
181 caggacaaga tcgtccttgg cgggcgcggt ggctactgct tcgaacataa cctgctcttc
241 atgcatgcgc tgaaggcgct tggtttcgag gtcggcgggt tggccgcgcg tgtgctctgg
301 ggccagagcg aggacgccat caccgcgcgc agccacatgc ttttgcgcgt cgagctcgac
361 ggccggacct acatcgccga tgtcggcttc ggcggcctga ccctgaccgc gccgctgctg
421 ctggaaccgg gccgcgaaca gaaaaccccg cacgagcctt tccgcatcgt cgaggccgac
481 gatcatttcc gcctgcaggc cgccatcggc ggcgactggc gctcgctcta ccgcttcgac
541 ctgcagccgc aatacgaagt cgattactcc gtcaccaatt acttcctgtc gaccagcccg
601 acatcgcatt tcctctcctc cgtcatcgcg gcacgcgccg cgccggaccg gcgctacgcg
661 ctgcggggaa accgcttgtc gatccatcac cttggcggcc gcaccgagca gacagagata
721 gccaccgccg ccgaccttgc cgacacgctg caaggcctgc tcggcatcat cattcccgac
781 cgcacggctt tcgaggccaa ggtgcgtgag acgaagatcg tggagaccaa cgcatga

fw atGGATCCatgaacgatg ctcctccctt c
rev atCCCGGG TCATGCGTTGGTCTCCACGATC

<blockquote data-quote="crystalizer" data-source="post: 3444691" data-attributes="member: 9193">Was not sure what you wanted... sry for that, misunderstanding.course ive planned sth out, i thought that you are going to do some fantasy work at your lab, and how do i know what you got in stock...mental masturbation looks different, belive me.Of course whole ORF.1) Mesorhizobium loti MAFF303099 chromosome, complete genomeNCBI Reference Sequence: NC_002678.2LOCUS&nbsp; &nbsp; &nbsp;&nbsp; NC_002678&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; 1554 bp&nbsp; &nbsp; DNA&nbsp; &nbsp;&nbsp; linear&nbsp;&nbsp; BCT 29-JUN-201mlr4653, aromatic-L-amino-acid decarboxylaseORIGIN&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 1 atgccgagga gatggcgctg tcgcgctcga cgcccggcgg ccagttcccg gcggcttgac&nbsp; &nbsp; &nbsp;&nbsp; 61 gggagcccgg tcatggacat cgcgcaggaa accgtcggca ccgaggaaac gctcgacccc&nbsp; &nbsp; &nbsp; 121 gccgactggg ccgcagtgca agccctgtcg caccaggtca tcgacgatgc cgtcgactat&nbsp; &nbsp; &nbsp; 181 ctcagcgaca tcagggaccg cccggcctgg cgcgagatgc cggcggaggt gcgagaagcc&nbsp; &nbsp; &nbsp; 241 ttcgcggcac ctctgccgcg atcgccggtg ccgctggccg ccgtctatga cgaggtctcg&nbsp; &nbsp; &nbsp; 301 cgcacggtga tgtcctatcc gatgggcaac atccatccgc gattctgggc ctggtatatg&nbsp; &nbsp; &nbsp; 361 gggtcgagca acttcaccgg cgcgctcggc gacttcctgg cggcaatcca gggttccaac&nbsp; &nbsp; &nbsp; 421 ctcggcggcg gcaaccacgc cgccgggctg atggacagcc aggtggtcaa ctggtgcaag&nbsp; &nbsp; &nbsp; 481 cagatgctgg gctttccggc ttccgcctcc ggcacgctgg tcagcggcgg ctcgatggcc&nbsp; &nbsp; &nbsp; 541 aacgtgatcg gcctgaccgt ggcgcgcaac gccaaggccg gcatcgacgt gcgcgagcac&nbsp; &nbsp; &nbsp; 601 ggcgtcgcgg cgatcgaaaa gccgctgcgc ttctatggct cggaccagat ccattcctgc&nbsp; &nbsp; &nbsp; 661 caccgcaagg caatggaagc acttggcctc ggcaaccggg cgctgcggcg catcccgacg&nbsp; &nbsp; &nbsp; 721 gatgccgacc tgcgcatcga cataccggcc ttgcgcgcgg cgatcgttga ggaccgcgaa&nbsp; &nbsp; &nbsp; 781 gccggtttca agccggcctg cgtcatcggc aatgccggca cggtcaacac cggcgcgatc&nbsp; &nbsp; &nbsp; 841 gacgatttgc gggcgcttgc caaactcgcg catgaggaag gcctatggtt ccatgtcgat&nbsp; &nbsp; &nbsp; 901 ggctgcatcg gcgcgctgat cgcgatcgcc cccgacaatg cgcatcgcgt tgccggcatc&nbsp; &nbsp; &nbsp; 961 gaatgggcgg attccgtcgc gctcgatccg cacaaatggc tgcatgcccc gttcgaggtc&nbsp; &nbsp;&nbsp; 1021 ggctgcgccc tggtcaggga tgccgtcgcc caccgccgga ccttcgcggt gacgccggaa&nbsp; &nbsp;&nbsp; 1081 tatctggaat cgacgccgcg cggccttgcc tccggcgaat ggctgcacga ttacggcctg&nbsp; &nbsp;&nbsp; 1141 cagacgtcgc gcggctttcg cgcgctgaaa gtctggatgg cgctgaagga acatggtgtc&nbsp; &nbsp;&nbsp; 1201 gacaaattcg gccgcctgat cgaccagaac atcgcgcaag cctcctatct cgccggggtg&nbsp; &nbsp;&nbsp; 1261 gtcgaggccg agccgctgat ggagctggcg acgtcgccga ccatcaacat cgtctgcttc&nbsp; &nbsp;&nbsp; 1321 cgctaccagc ccggcgtcac cggcgaggcg ctgaaggtgc tcaacaccga gatcatgctg&nbsp; &nbsp;&nbsp; 1381 cggctgcagg aacagggcat cgccgtcctt tccgacacta ccgtgcatgg cgaacactgg&nbsp; &nbsp;&nbsp; 1441 ctgcgcgtgg cgatcgccaa ccaccgcaca aggcgcgagg acctcgacct tctggtcgag&nbsp; &nbsp;&nbsp; 1501 gaaacggtgc ggttgggacg tgagatcgca gcagaaggct ccccccggaa atagfw&nbsp;&nbsp; atGGATCC atgccgagga gatggcgctg&nbsp; &nbsp; &nbsp; &nbsp; rev&nbsp; atCCCGGG CTATTTCCGGGGGGAGCCTT&nbsp; &nbsp; &nbsp; reverse primer would suck. not equally distributed. better primer MUST be outside ORF.2) MO as above, gene=&quot;mlr4870&quot;, arylamine N-acetyltransferaseORIGIN&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 1 atgaacgatg ctcctccctt cgacctcgac gcctatctcg ctcgcatcgg ttacaccggt&nbsp; &nbsp; &nbsp;&nbsp; 61 ccgcgcaacg cgtcgctgga tacgctgaag gcgctgcatt tcgcgcatcc gcaggcgatc&nbsp; &nbsp; &nbsp; 121 cccttcgaga acatcgatcc gtttcttggt cgcccggtcc ggctcgacct tgccgcgctg&nbsp; &nbsp; &nbsp; 181 caggacaaga tcgtccttgg cgggcgcggt ggctactgct tcgaacataa cctgctcttc&nbsp; &nbsp; &nbsp; 241 atgcatgcgc tgaaggcgct tggtttcgag gtcggcgggt tggccgcgcg tgtgctctgg&nbsp; &nbsp; &nbsp; 301 ggccagagcg aggacgccat caccgcgcgc agccacatgc ttttgcgcgt cgagctcgac&nbsp; &nbsp; &nbsp; 361 ggccggacct acatcgccga tgtcggcttc ggcggcctga ccctgaccgc gccgctgctg&nbsp; &nbsp; &nbsp; 421 ctggaaccgg gccgcgaaca gaaaaccccg cacgagcctt tccgcatcgt cgaggccgac&nbsp; &nbsp; &nbsp; 481 gatcatttcc gcctgcaggc cgccatcggc ggcgactggc gctcgctcta ccgcttcgac&nbsp; &nbsp; &nbsp; 541 ctgcagccgc aatacgaagt cgattactcc gtcaccaatt acttcctgtc gaccagcccg&nbsp; &nbsp; &nbsp; 601 acatcgcatt tcctctcctc cgtcatcgcg gcacgcgccg cgccggaccg gcgctacgcg&nbsp; &nbsp; &nbsp; 661 ctgcggggaa accgcttgtc gatccatcac cttggcggcc gcaccgagca gacagagata&nbsp; &nbsp; &nbsp; 721 gccaccgccg ccgaccttgc cgacacgctg caaggcctgc tcggcatcat cattcccgac&nbsp; &nbsp; &nbsp; 781 cgcacggctt tcgaggccaa ggtgcgtgag acgaagatcg tggagaccaa cgcatgafw atGGATCCatgaacgatg ctcctccctt c&nbsp;&nbsp; rev atCCCGGG&nbsp; &nbsp; TCATGCGTTGGTCTCCACGATC</blockquote>

[QUOTE="crystalizer, post: 3444691, member: 9193"] Was not sure what you wanted... sry for that, misunderstanding. course ive planned sth out, i thought that you are going to do some fantasy work at your lab, and how do i know what you got in stock...mental masturbation looks different, belive me. Of course whole ORF. 1) Mesorhizobium loti MAFF303099 chromosome, complete genome NCBI Reference Sequence: NC_002678.2 LOCUS NC_002678 1554 bp DNA linear BCT 29-JUN-201 mlr4653, aromatic-L-amino-acid decarboxylase ORIGIN 1 atgccgagga gatggcgctg tcgcgctcga cgcccggcgg ccagttcccg gcggcttgac 61 gggagcccgg tcatggacat cgcgcaggaa accgtcggca ccgaggaaac gctcgacccc 121 gccgactggg ccgcagtgca agccctgtcg caccaggtca tcgacgatgc cgtcgactat 181 ctcagcgaca tcagggaccg cccggcctgg cgcgagatgc cggcggaggt gcgagaagcc 241 ttcgcggcac ctctgccgcg atcgccggtg ccgctggccg ccgtctatga cgaggtctcg 301 cgcacggtga tgtcctatcc gatgggcaac atccatccgc gattctgggc ctggtatatg 361 gggtcgagca acttcaccgg cgcgctcggc gacttcctgg cggcaatcca gggttccaac 421 ctcggcggcg gcaaccacgc cgccgggctg atggacagcc aggtggtcaa ctggtgcaag 481 cagatgctgg gctttccggc ttccgcctcc ggcacgctgg tcagcggcgg ctcgatggcc 541 aacgtgatcg gcctgaccgt ggcgcgcaac gccaaggccg gcatcgacgt gcgcgagcac 601 ggcgtcgcgg cgatcgaaaa gccgctgcgc ttctatggct cggaccagat ccattcctgc 661 caccgcaagg caatggaagc acttggcctc ggcaaccggg cgctgcggcg catcccgacg 721 gatgccgacc tgcgcatcga cataccggcc ttgcgcgcgg cgatcgttga ggaccgcgaa 781 gccggtttca agccggcctg cgtcatcggc aatgccggca cggtcaacac cggcgcgatc 841 gacgatttgc gggcgcttgc caaactcgcg catgaggaag gcctatggtt ccatgtcgat 901 ggctgcatcg gcgcgctgat cgcgatcgcc cccgacaatg cgcatcgcgt tgccggcatc 961 gaatgggcgg attccgtcgc gctcgatccg cacaaatggc tgcatgcccc gttcgaggtc 1021 ggctgcgccc tggtcaggga tgccgtcgcc caccgccgga ccttcgcggt gacgccggaa 1081 tatctggaat cgacgccgcg cggccttgcc tccggcgaat ggctgcacga ttacggcctg 1141 cagacgtcgc gcggctttcg cgcgctgaaa gtctggatgg cgctgaagga acatggtgtc 1201 gacaaattcg gccgcctgat cgaccagaac atcgcgcaag cctcctatct cgccggggtg 1261 gtcgaggccg agccgctgat ggagctggcg acgtcgccga ccatcaacat cgtctgcttc 1321 cgctaccagc ccggcgtcac cggcgaggcg ctgaaggtgc tcaacaccga gatcatgctg 1381 cggctgcagg aacagggcat cgccgtcctt tccgacacta ccgtgcatgg cgaacactgg 1441 ctgcgcgtgg cgatcgccaa ccaccgcaca aggcgcgagg acctcgacct tctggtcgag 1501 gaaacggtgc ggttgggacg tgagatcgca gcagaaggct ccccccggaa atag fw atGGATCC atgccgagga gatggcgctg rev atCCCGGG CTATTTCCGGGGGGAGCCTT reverse primer would suck. not equally distributed. better primer MUST be outside ORF. 2) MO as above, gene="mlr4870", arylamine N-acetyltransferase ORIGIN 1 atgaacgatg ctcctccctt cgacctcgac gcctatctcg ctcgcatcgg ttacaccggt 61 ccgcgcaacg cgtcgctgga tacgctgaag gcgctgcatt tcgcgcatcc gcaggcgatc 121 cccttcgaga acatcgatcc gtttcttggt cgcccggtcc ggctcgacct tgccgcgctg 181 caggacaaga tcgtccttgg cgggcgcggt ggctactgct tcgaacataa cctgctcttc 241 atgcatgcgc tgaaggcgct tggtttcgag gtcggcgggt tggccgcgcg tgtgctctgg 301 ggccagagcg aggacgccat caccgcgcgc agccacatgc ttttgcgcgt cgagctcgac 361 ggccggacct acatcgccga tgtcggcttc ggcggcctga ccctgaccgc gccgctgctg 421 ctggaaccgg gccgcgaaca gaaaaccccg cacgagcctt tccgcatcgt cgaggccgac 481 gatcatttcc gcctgcaggc cgccatcggc ggcgactggc gctcgctcta ccgcttcgac 541 ctgcagccgc aatacgaagt cgattactcc gtcaccaatt acttcctgtc gaccagcccg 601 acatcgcatt tcctctcctc cgtcatcgcg gcacgcgccg cgccggaccg gcgctacgcg 661 ctgcggggaa accgcttgtc gatccatcac cttggcggcc gcaccgagca gacagagata 721 gccaccgccg ccgaccttgc cgacacgctg caaggcctgc tcggcatcat cattcccgac 781 cgcacggctt tcgaggccaa ggtgcgtgag acgaagatcg tggagaccaa cgcatga fw atGGATCCatgaacgatg ctcctccctt c rev atCCCGGG TCATGCGTTGGTCTCCACGATC [/QUOTE]