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Hey! First of all THX for all this theoretical imput and for keeping this topic alive...

Reasearch is like Infundibulum says... sometimes it takes more time than expected. For insance i recieved in the beginning of 2011 a small project - 2 months maximum was planned. ... here we are two years later, with the particular project still not finished....

[USER=769]@Infundibulum[/USER]
INMT and TDC shoud be tagged, yeah... isn't the simple his tag eough? purify it and end up with correct bands (SDS-PAGE)...? or do you really thinking about westernblotting it?

@ MR. M.S cell/molecular biology - whitebread, i agree with endlessness and Infundibulum. they both gave a very statisfying replies - i have nothing else to add...

@ adoro : NICE, very interesting input. when i find some more time, i will go through your thread.... i have to agree with benzyme and Infundibulum, enzyme purifications are off the table ...
i worked with tabacco in cell cutre... it wont work at home...you are going to contaminate everything before it even starts growing...
the bioreactor pipeline is also great, of course we will use one but FiRST we need some basic research, about which bacteria/enzyme is doing the job, at which conditios etc. and then we can think about pipelining it...

BTW - you all convinced me of trying it in yeast (if i ever try it...) - even if the colis would grow faster... for home usage yeast is more convinient... some nice stock in the freezer, put it in culture and harvest couple days later... it will be still faster than trying to find some mhrb ...

<blockquote data-quote="crystalizer" data-source="post: 3445722" data-attributes="member: 9193">Hey! First of all THX for all this theoretical imput and for keeping this topic alive...Reasearch is like Infundibulum says... sometimes it takes more time than expected. For insance i recieved in the beginning of 2011 a small project - 2 months maximum was planned. ... here we are two years later, with the particular project still not finished.... [USER=769]@Infundibulum[/USER]INMT and TDC shoud be tagged, yeah... isn&#039;t the simple his tag eough?&nbsp; purify it and end up with correct bands (SDS-PAGE)...? or do you really thinking about westernblotting it?@ MR. M.S cell/molecular biology - whitebread, i agree with endlessness and Infundibulum. they both gave a very statisfying replies&nbsp; - i have nothing else to add...@ adoro : NICE, very interesting input. when i find some more time, i will go through your thread.... i have to agree with benzyme and Infundibulum, enzyme purifications are off the table ...i worked with tabacco in cell cutre... it wont work at home...you are going to contaminate everything before it even starts growing...the bioreactor pipeline is also great, of course we will use one <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite2" alt=";)" title="Wink&nbsp; &nbsp; ;)" loading="lazy" data-shortname=";)" /> but FiRST we need some basic research, about which bacteria/enzyme is doing the job, at which conditios etc. and then we can think about pipelining it...&nbsp; BTW - you all convinced me of trying it in yeast (if i ever try it...) - even if the colis would grow faster...&nbsp; for home usage yeast is more convinient... some nice stock in the freezer, put it in culture and harvest couple days later... it will be still faster than trying to find some&nbsp; mhrb ...</blockquote>

[QUOTE="crystalizer, post: 3445722, member: 9193"] Hey! First of all THX for all this theoretical imput and for keeping this topic alive... Reasearch is like Infundibulum says... sometimes it takes more time than expected. For insance i recieved in the beginning of 2011 a small project - 2 months maximum was planned. ... here we are two years later, with the particular project still not finished.... [USER=769]@Infundibulum[/USER] INMT and TDC shoud be tagged, yeah... isn't the simple his tag eough? purify it and end up with correct bands (SDS-PAGE)...? or do you really thinking about westernblotting it? @ MR. M.S cell/molecular biology - whitebread, i agree with endlessness and Infundibulum. they both gave a very statisfying replies - i have nothing else to add... @ adoro : NICE, very interesting input. when i find some more time, i will go through your thread.... i have to agree with benzyme and Infundibulum, enzyme purifications are off the table ... i worked with tabacco in cell cutre... it wont work at home...you are going to contaminate everything before it even starts growing... the bioreactor pipeline is also great, of course we will use one ;) but FiRST we need some basic research, about which bacteria/enzyme is doing the job, at which conditios etc. and then we can think about pipelining it... BTW - you all convinced me of trying it in yeast (if i ever try it...) - even if the colis would grow faster... for home usage yeast is more convinient... some nice stock in the freezer, put it in culture and harvest couple days later... it will be still faster than trying to find some mhrb ... [/QUOTE]