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Sorry to resurrect this old thread, but the pathway map above is very intriguing. According to the map, it is entirely possible for yeast to make N,N-DMT without any genetic modifications. Of course, the quantities of DMT are likely quite low, if any.


According to the map, tryptamine is used as a precursor in the production of N-hydroxy-tryptamine and indole-3-acetaldehyde. What if one were to knock out the genes responsible for those products (1.1.4.1.3- and 1.4.3.22/1.4.3.4)? If those knockouts do not prove to be lethal, it would elevate tryptamine levels in the cell allowing it to be used exclusively by 2.1.1.49.


This is highly speculative, but I'm interested in the community's take on it.


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