pardon me. I should have been more specific. Haven't practiced this hobby inna while..

It was incoculated once and nothing happened, so the second time sporesoluion was deposited all over the dish. Obviously something went wrong on each inoculation point. Which leads me to think it had to be the spore solution itself. But then again its highly unlikely that it happens in five different syringes. Like you say, there could be dozen of reasons. Next test will be with fresh needles and only one inoculation point.

Thank you!