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Thanks:thumb_up:I'm about to embark on my first syrian rue extraction, and may use this method!Couple questions, is the freeze/thaw just for cell lysing, and if so, couldn't it be replaced with grinding the seeds?Also, not trying to be picky, and maybe i read wrong, am misinformed etc, but wouldn't it be an acetate, not HCl in step 5b?Good work man, you were promoted before i got a chance to vote you up, so here's a :thumb_up:
Thanks:thumb_up:
I'm about to embark on my first syrian rue extraction, and may use this method!
Couple questions, is the freeze/thaw just for cell lysing, and if so, couldn't it be replaced with grinding the seeds?
Also, not trying to be picky, and maybe i read wrong, am misinformed etc, but wouldn't it be an acetate, not HCl in step 5b?
Good work man, you were promoted before i got a chance to vote you up, so here's a :thumb_up: