The amount of freebase harmaloids you get will depend on your extraction methods, and the seeds themselves.
The only way to figure out how potent your seeds are, is to either go by how strong it feels, when made into a tea, or (best way imho) do a proper acid/base/manske/base extraction. By that, i mean make a tea, filter it good, base the alk's out, filter them out, manske that, base the manske'd hcl alks once you dissolve them in hot distilled water again.
That will give you pure freebase harmaloids from rue, and in turn an accurate % by weight yeild, assuming you perform the extraction without losses/waste of alkaloids.
No other way to tell really.
The seeds themselves vary, as do peoples yields due to extraction method/skill.
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