Kambogahuascanga
Rising Star
I finally recieved word about the way to make Iboga go vroom vroom in terms of growing it. I left out the person's name I am talking to. This is very encouraging to me!
Sorry I haven't gotten back to you sooner, I just spent the last 4 days back in ________ I hybridizing magnolias with several people from around the area so it was a busy couple of days.
BUt yes I have talked a lot with _______, and she was very gracious in her help with my efforts to work with this plant. I haven't talked to her in a while so I should drop her a line and to say hi.
So what mainly has been happening is Dr______________ (Who is the younger brother of _______________________ of the 80's) has been working with ____________ at the University of ______________ with propagating Iboga and working to put the plants into tissue culture, which is pretty much a plantlet in a small, jar, but has an ideal environment to grow to maximize the growth on the plant. Tissue culture jars are completely sterile inside so no other fungi or bacteria can contaminate them and the media that the plantlets reside in are filled with all the basic sugars and nutrience that the plants need to grow optimally. A picture to kind of get and idea of what I am talking about (http://www.thailandinone.com/dotcom/images/relate/anthurium/alibaba/big/tissue_culture1.jpg)
With using tissue culture this allows us to manipulate the plants in ways that we couldnt otherwise in the outside world because of too many stress pressures put on by both other organisms and the environment. What our plan is to do is infect the young plantlets of iboga that we already have in culture, with a bacteria to induce hairy root culture. (Hairy root culture - Wikipedia) Explains it better and so I don't have to type as much. Basically the hairy roots ofthe iboga plant are growing very fast and very fiborious roots, unlike the clumpy slow growing roots of the normal iboga plant. (http://img.springerimages.com/Image...diaObjects/WATER_11033_2010_363_Fig1_HTML.jpg) That is a picture of how hairy root culture is induced. As you can see a lot of roots are produced very quickly. (Bottom two pictures) . Within those roots ibogaine is still being stored and made, but within a matter of months and not years, we can have enough root buildup in the jar to have produced the same amount of ibogaine that a very old plant would have done. The ibogaine is then just extracted straight from the roots and the media around it when the plants are ready to be harvested and it is very easy to get the pure ibogaine right infront of us. Thus saving lots of time in the process, this is what I explained to ________ as that in a matter around 6 months produce the same amount of ibogaine that an adult plant can make. Thats basically the research that I will be doing for my gradschool in a year when I go up to ______________________.
So tissue cultire can also be used to mass propigate a plant. Lets say if there was a particularly good plant of iboga that produced high levels of ibogaine and you wanted a lot more of those plants, you can get a few plantlets into the sterile enviroment, then just keep sub-culturing it and producing as many plantlets of it as you want. You can make one little plant into millions of little plants, just depends upon how much you want.
With the hybridizing of iboga it has been mostly for me a lot of thinking and not being able to do much work. This is something I want to do on my own time, it wouldn't be a part of my research at UBC. With me doing my undergrad in the USA iboga is illegal here so a lot of my breeding work has not been ableto be produced yet. But mainly I'll explain my ideas. For being able to farm iboga outside of a lab, on a real farm, iboga is really slow and kind of temperamental with temperatures and such. So I want to try to cross it with other plants closely related to it to create hybrids that are a lot faster growing, hardier, and a lot easier to propagate. I want to cross it with other species within its own family (apocynaceae) too add vigor to the plants with the likes of the tabernaemontana genus, very closely related to the tabernanthe genus and also with vinca genus to add cold hardiness. Vinca grows up into the northern range and can survive temperatures well below 0 degrees F. But there is a lot of work to be done there with getting pollen of iboga and then actually getting the crosses to take, right now those are way back in the pipeline.
So those are some basic explanations that are going on, I know I can have a lot more to write and I can add upon this info in future emails. Speaking of emails, do you have an email address, because I can try to pass you onto _______ so we can see how we each can help eachother accomplish our goals. Thank you so much for getting a hold of me and I hope to hear from you soon.
Take care,
Sorry I haven't gotten back to you sooner, I just spent the last 4 days back in ________ I hybridizing magnolias with several people from around the area so it was a busy couple of days.
BUt yes I have talked a lot with _______, and she was very gracious in her help with my efforts to work with this plant. I haven't talked to her in a while so I should drop her a line and to say hi.
So what mainly has been happening is Dr______________ (Who is the younger brother of _______________________ of the 80's) has been working with ____________ at the University of ______________ with propagating Iboga and working to put the plants into tissue culture, which is pretty much a plantlet in a small, jar, but has an ideal environment to grow to maximize the growth on the plant. Tissue culture jars are completely sterile inside so no other fungi or bacteria can contaminate them and the media that the plantlets reside in are filled with all the basic sugars and nutrience that the plants need to grow optimally. A picture to kind of get and idea of what I am talking about (http://www.thailandinone.com/dotcom/images/relate/anthurium/alibaba/big/tissue_culture1.jpg)
With using tissue culture this allows us to manipulate the plants in ways that we couldnt otherwise in the outside world because of too many stress pressures put on by both other organisms and the environment. What our plan is to do is infect the young plantlets of iboga that we already have in culture, with a bacteria to induce hairy root culture. (Hairy root culture - Wikipedia) Explains it better and so I don't have to type as much. Basically the hairy roots ofthe iboga plant are growing very fast and very fiborious roots, unlike the clumpy slow growing roots of the normal iboga plant. (http://img.springerimages.com/Image...diaObjects/WATER_11033_2010_363_Fig1_HTML.jpg) That is a picture of how hairy root culture is induced. As you can see a lot of roots are produced very quickly. (Bottom two pictures) . Within those roots ibogaine is still being stored and made, but within a matter of months and not years, we can have enough root buildup in the jar to have produced the same amount of ibogaine that a very old plant would have done. The ibogaine is then just extracted straight from the roots and the media around it when the plants are ready to be harvested and it is very easy to get the pure ibogaine right infront of us. Thus saving lots of time in the process, this is what I explained to ________ as that in a matter around 6 months produce the same amount of ibogaine that an adult plant can make. Thats basically the research that I will be doing for my gradschool in a year when I go up to ______________________.
So tissue cultire can also be used to mass propigate a plant. Lets say if there was a particularly good plant of iboga that produced high levels of ibogaine and you wanted a lot more of those plants, you can get a few plantlets into the sterile enviroment, then just keep sub-culturing it and producing as many plantlets of it as you want. You can make one little plant into millions of little plants, just depends upon how much you want.
With the hybridizing of iboga it has been mostly for me a lot of thinking and not being able to do much work. This is something I want to do on my own time, it wouldn't be a part of my research at UBC. With me doing my undergrad in the USA iboga is illegal here so a lot of my breeding work has not been ableto be produced yet. But mainly I'll explain my ideas. For being able to farm iboga outside of a lab, on a real farm, iboga is really slow and kind of temperamental with temperatures and such. So I want to try to cross it with other plants closely related to it to create hybrids that are a lot faster growing, hardier, and a lot easier to propagate. I want to cross it with other species within its own family (apocynaceae) too add vigor to the plants with the likes of the tabernaemontana genus, very closely related to the tabernanthe genus and also with vinca genus to add cold hardiness. Vinca grows up into the northern range and can survive temperatures well below 0 degrees F. But there is a lot of work to be done there with getting pollen of iboga and then actually getting the crosses to take, right now those are way back in the pipeline.
So those are some basic explanations that are going on, I know I can have a lot more to write and I can add upon this info in future emails. Speaking of emails, do you have an email address, because I can try to pass you onto _______ so we can see how we each can help eachother accomplish our goals. Thank you so much for getting a hold of me and I hope to hear from you soon.
Take care,
