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Hi guys,Today I made a few cakes using the PF Tek.As stated before sterilizing the jars, I turned open the lid of each jar a bit.After sterilization I closed each of them, which caused a major problem while inoculating them in my glove box: when putting the tip of the syringe through the hole (which were covered with duck tape) in the lid it sucked out almost half of the liquid into 1 jar I am not sure, but probably there was a vacuum of some kind in the jars that caused this, any tips on what I forgot to do? Should I have opened the lids instead of closing them directly. What I did for the other jars is pin the holes with a needle first and that seemed to work...Any other suggestions? I feel a bit dumb cause of this. Hopefully the other jars will still form mycelium.
Hi guys,
Today I made a few cakes using the PF Tek.
As stated before sterilizing the jars, I turned open the lid of each jar a bit.
After sterilization I closed each of them, which caused a major problem while inoculating them in my glove box: when putting the tip of the syringe through the hole (which were covered with duck tape) in the lid it sucked out almost half of the liquid into 1 jar
I am not sure, but probably there was a vacuum of some kind in the jars that caused this, any tips on what I forgot to do? Should I have opened the lids instead of closing them directly. What I did for the other jars is pin the holes with a needle first and that seemed to work...
Any other suggestions? I feel a bit dumb cause of this. Hopefully the other jars will still form mycelium.