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Hi guys,


Today I made a few cakes using the PF Tek.


As stated before sterilizing the jars, I turned open the lid of each jar a bit.

After sterilization I closed each of them, which caused a major problem while inoculating them in my glove box: when putting the tip of the syringe through the hole (which were covered with duck tape) in the lid it sucked out almost half of the liquid into 1 jar :(


I am not sure, but probably there was a vacuum of some kind in the jars that caused this, any tips on what I forgot to do? Should I have opened the lids instead of closing them directly. What I did for the other jars is pin the holes with a needle first and that seemed to work...


Any other suggestions? I feel a bit dumb cause of this. Hopefully the other jars will still form mycelium.


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