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Alright, here is a second attempt using Na+ ion exchange resin.Started with hot filtered vinegar tea (120g fresh mushrooms, 45m seep). This time I added a lot of Na+ exchange resin (280g) and mixed it all up well for a few minutes by shaking. Samples were taken before/after ion exchange for blueing (will post that later after they have time to oxydize).Resin was recovered and rinsed with water. First rinses where muddy, last rinses were clear as seen in the two leftmost jars in the image below. These rinses will also be aged and checked for oxydation.The resin was rinsed with 75% ethanol and the alcohol remained clear.Next, the resin was pulled with 75% ethanol by adding some drops of ammonia (pH shot up to 10.6, I hope I did not overdo it; no blueing was observed which may be a godd sign) . The alcohol turned tawny under room lighting and fluorescent under UV, indicating that material was being pulled from the resin. The resin was pulled two more times with fresh ethanol (pH remained above 9 on the recovered alcohol). By the third and last pull the twanyness/fluorescence was barely noticeable. The alcohol pulls were combined and made acidic with 1/4 teaspoon of tartaric acid (pH ~ 4). The resulting acidified basic ethanol pull is the rightmost jar of the picture below.Next the tawny alcohol will be dried (expecting crystalline result) and bioassyed. If potent, the powder will be cleaned up (dry acetone and maybe IPA/naphtha) and sent for analysis.
Alright, here is a second attempt using Na+ ion exchange resin.
Started with hot filtered vinegar tea (120g fresh mushrooms, 45m seep). This time I added a lot of Na+ exchange resin (280g) and mixed it all up well for a few minutes by shaking. Samples were taken before/after ion exchange for blueing (will post that later after they have time to oxydize).
Resin was recovered and rinsed with water. First rinses where muddy, last rinses were clear as seen in the two leftmost jars in the image below. These rinses will also be aged and checked for oxydation.
The resin was rinsed with 75% ethanol and the alcohol remained clear.
Next, the resin was pulled with 75% ethanol by adding some drops of ammonia (pH shot up to 10.6, I hope I did not overdo it; no blueing was observed which may be a godd sign) . The alcohol turned tawny under room lighting and fluorescent under UV, indicating that material was being pulled from the resin. The resin was pulled two more times with fresh ethanol (pH remained above 9 on the recovered alcohol). By the third and last pull the twanyness/fluorescence was barely noticeable. The alcohol pulls were combined and made acidic with 1/4 teaspoon of tartaric acid (pH ~ 4). The resulting acidified basic ethanol pull is the rightmost jar of the picture below.
Next the tawny alcohol will be dried (expecting crystalline result) and bioassyed. If potent, the powder will be cleaned up (dry acetone and maybe IPA/naphtha) and sent for analysis.