Here is an update.

280g of fresh mushrooms were extracted by seeping in plain hot water for 10 minutes with intermittent squeezimg with a potato masher. This tea was filtered.

To the filtered tea, half a cup of Na+ strong cation resin (pictured here) wass added. Tea and resin was shaken multiple times over a 30 minute period. Water was filtered out using a nylon mesh.

The cation resin was repeatedly washed with water until it ran clear (5 times).

75% everclear was added to to the resin. The everclear remained clear at this point. To this, baking soda was added until a pH of 9.1 was reached. At this point, the everclear became tawny under normal light and fluorescent under UV.

The twany everclear was filtered and to this 2g of HPBCD was added and stirred well in the hopes that it would complex with and protect any actives during subsequent steps. This is because in all previous testing, activity was mimal for all dried product with one possibility for this being damage from oxygen while drying.

At this point 250ml of tawny everclear with baking soda and HPBCD were obtained.

100ml were reserved for a later bioassay. This is why baking soda was used and not ammonia, so that a bioassay could be done without evaporating.

50ml were dryed to obtain 900mg of white powder containing HPBCD, baking powder, and mushroom material. This is reserved for later bioassay.

100ml of this were diluted up to 900ml using acetone and put in the freezer. Material began to precipitate out. After two days in the freezer, the solution was filtered and evaporated. The remaining material after evaporation was scraped up relatively easily to form a crystalline fluorescent powder. This has been reserved for bioassay. There are some visible tawny "hot spots" in the powder which will be mixed in before bioassay. Pictures after drying and after scraping in normal and UV light are below.

Next, bioassys will be done. They could all be negative - I'll report results as they come in.