Quick update.

10g of mushrooms and 3/4 teaspoon of Vit C where steeped in 70C water for 1h to try to convert psilocybin into psilocinH+ and move it into the water. This tea was filtered (final pH 4.35) and shaken with Na cation exchange resin in a Mason jar for 10 minutes. The tea was then filtered out and saved (plan to bioasy this, should not be active if both the phosphatase enzymes and the cation exchange resin worked so that actives ended up as psilocinH+ binded to the resin).

The beads were washed with water until water ran clear (normal and UV light). Then the beads were extracted (aka eluted) with baking soda water (~pH 8.5) which became tawny and fluorescent under UV. After this elution these beads (calling them mother resin beads) were set aside for other tests

Followed downwardsfromzero's advice and bioassayed 1/5th of this basified water (neutralized it with vitamin C first to make a delicious effervescent drink), unfortunately it was not active.

So where are the actives? I took small samples of the mother resin and tried different tests. One was elutimg with ammonia and ethyl acetate (MEK substitute at the hardware store). The acetate became cloudy. The water was also tawny, and then started changing color to green, indicating that something new was being eluted.

So maybe the baking soda water was not strong enough to elute psilocin?

As mentioned before, will test the original tea that was treated with the resin to see if any actives remain there.

Ideas welcome, more tests to follow, including boiling the water at the end to denatured proteins.