Interesting, I put some of the mother ion from the previous post in alkaline water again and also in HPBCD (no color change after shaking) and then ran it in the microwave for one minute, it boiled for 20s or so. The water solution came out cloudy, so something happened.

Does the temperature and/or microwave help elution?

I wonder if at higher temperature it is easier to pull psilocin out off the resin at mild pH (if indeed it is trapped in there). Water solubility changes a lot for psilocin from reading the info out there, so it's plausible I guess. That would be ideal since a bunch of non active twany stuff was eluted already at room temp (from the bioassay in the previous post).

Edit: Attaching some literature on the subject. Though I don't quite understand it, it looks like temperature can change how things elute.