Members of the previous forum can retrieve their temporary password here, (login and check your PM).

Reply to thread

Message

Alright, I drank 1/10 of of the 10g mushroom filtered tea that had been steeped in 500ml of water with 3/4 tsp of Vit C at 70C for 1h (final pH 4.3) and then treated with cation resin. The tea had been kept in the fridge (wrapped in aluminum foil in a mason jar) for a few days.

Result: Some nervous initial energy, but no significant effects. I fell asleep quickly which is not possible for me at all with mushrooms. The tea had all the funky taste of shrooms though

I think this indicates the psilocybin did convert into psilocinH+ during the acidic 70C 1h water steep and was subsequently absorbed by the ion exchange cation resin.

Based on the results from trying to recover (elute) the psilocin, it seems that room temp baking soda water does not elute it - but it does elute something that does not seem active. Maybe just water soluble proteins with -OH groups.

Several more tests have been done and based on color changes (towards a greenish blue) other systems acould be eluting psilocin (for example ethyl acetate and ammonia, or microwaved hot baking soda water).

Out thhese elution options tested, one that I'm going to focus on more is baking soda + 75% everclear. A promising green fluorescence clear liquid was eluted by this system from the mother beads (which had already previously been washed until baking soda water ran clear).

I have a sample of this 75% ethanol elute that was neutralized with VitC quickly after elution Hpbcd was also added before neutralizing it. I'm hoping the goods are in it and plan to bioasy this.

If bioassay of this elute is positive, will try to dry it in air to see HPBCD can protect psilocin. If activity is lost during air dry will try to crash a hypothetical hpbcd complex (with acetone). If that fails, will invest a couple hundred bucks in the equipment to lyophilize (freeze dry) the (hopefully active) neutralized elute + HPBCD.

Edit:Added a couple articles discussing HPBCD complexation of curcumin (which also has an -OH group that degrades in Alkaline conditions). To make a complex they did basify and then quickly neutralized curcumin in the presence of HPBCD. Above something similar was don't, but the HPBCD was added after the ion resin pull, instead of before the drug is put in the Alkaline solution. Next time I'll modify the order, so that HPBCD can complex at t=0.

<blockquote data-quote="Loveall" data-source="post: 3573870" data-attributes="member: 20">Alright, I drank 1/10 of of the 10g mushroom filtered tea that had been steeped in 500ml of water with 3/4 tsp of Vit C at 70C for 1h (final pH 4.3) and then treated with cation resin. The tea had been kept in the fridge (wrapped in aluminum foil in a mason jar) for a few days. Result: Some nervous initial energy, but no significant effects. I fell asleep quickly which is not possible for me at all with mushrooms. The tea had all the funky taste of shrooms though <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite7" alt=":p" title="Stick out tongue&nbsp; &nbsp; :p" loading="lazy" data-shortname=":p" /> I think this indicates the psilocybin did convert into psilocinH+ during the acidic 70C 1h water steep and was subsequently absorbed by the ion exchange cation resin. Based on the results from trying to recover (elute) the psilocin, it seems that room temp baking soda water does not elute it - but it does elute something that does not seem active. Maybe just water soluble proteins with -OH groups. Several more tests have been done and based on color changes (towards a greenish blue) other systems acould be eluting psilocin (for example ethyl acetate and ammonia, or microwaved hot baking soda water). Out thhese elution options tested, one that I&#039;m going to focus on more is baking soda + 75% everclear. A promising green fluorescence clear liquid was eluted by this system from the mother beads (which had already previously been washed until baking soda water ran clear).I have a sample of this 75% ethanol elute that was neutralized with VitC quickly after elution Hpbcd was also added before neutralizing it. I&#039;m hoping the goods are in it and plan to bioasy this. If bioassay of this elute is positive, will try to dry it in air to see HPBCD can protect psilocin. If activity is lost during air dry will try to crash a hypothetical hpbcd complex (with acetone). If that fails, will invest a couple hundred bucks in the equipment to lyophilize (freeze dry) the (hopefully active) neutralized elute + HPBCD.Edit:Added a couple articles discussing HPBCD complexation of curcumin (which also has an -OH group that degrades in Alkaline conditions). To make a complex they did basify and then quickly neutralized curcumin in the presence of HPBCD. Above something similar was don&#039;t, but the HPBCD was added after the ion resin pull, instead of before the drug is put in the Alkaline solution. Next time I&#039;ll modify the order, so that HPBCD can complex at t=0.</blockquote>

[QUOTE="Loveall, post: 3573870, member: 20"] Alright, I drank 1/10 of of the 10g mushroom filtered tea that had been steeped in 500ml of water with 3/4 tsp of Vit C at 70C for 1h (final pH 4.3) and then treated with cation resin. The tea had been kept in the fridge (wrapped in aluminum foil in a mason jar) for a few days. Result: Some nervous initial energy, but no significant effects. I fell asleep quickly which is not possible for me at all with mushrooms. The tea had all the funky taste of shrooms though :p I think this indicates the psilocybin did convert into psilocinH+ during the acidic 70C 1h water steep and was subsequently absorbed by the ion exchange cation resin. Based on the results from trying to recover (elute) the psilocin, it seems that room temp baking soda water does not elute it - but it does elute something that does not seem active. Maybe just water soluble proteins with -OH groups. Several more tests have been done and based on color changes (towards a greenish blue) other systems acould be eluting psilocin (for example ethyl acetate and ammonia, or microwaved hot baking soda water). Out thhese elution options tested, one that I'm going to focus on more is [b]baking soda + 75% everclear[/b]. A promising green fluorescence clear liquid was eluted by this system from the mother beads (which had already previously been washed until baking soda water ran clear). I have a sample of this 75% ethanol elute that was neutralized with VitC quickly after elution Hpbcd was also added before neutralizing it. I'm hoping the goods are in it and plan to bioasy this. If bioassay of this elute is positive, will try to dry it in air to see HPBCD can protect psilocin. If activity is lost during air dry will try to crash a hypothetical hpbcd complex (with acetone). If that fails, will invest a couple hundred bucks in the equipment to lyophilize (freeze dry) the (hopefully active) neutralized elute + HPBCD. Edit:Added a couple articles discussing HPBCD complexation of curcumin (which also has an -OH group that degrades in Alkaline conditions). To make a complex they did basify and then quickly neutralized curcumin in the presence of HPBCD. Above something similar was don't, but the HPBCD was added after the ion resin pull, instead of before the drug is put in the Alkaline solution. Next time I'll modify the order, so that HPBCD can complex at t=0. [/QUOTE]