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Result: whatever eluted with alcohol and baking soda was not active.Among the many possible issues I think I may be having trouble eluting the beads. Bellow is a picture of the beads in a baking soda solution after a few days. Looks like something that is still stuck to the beads is degrading.Gonna try to elute again with washing soda.Another possible issue is that the psilocin is binded to a protein and that protein is stuck hard to the ion resin. Maybe denaturing proteins with heat at the end ot the tea steeping step could help (boil at 100C for a few minutes)?Anyway, will keep on trying but ideas welcome.
Result: whatever eluted with alcohol and baking soda was not active.
Among the many possible issues I think I may be having trouble eluting the beads. Bellow is a picture of the beads in a baking soda solution after a few days. Looks like something that is still stuck to the beads is degrading.
Gonna try to elute again with washing soda.
Another possible issue is that the psilocin is binded to a protein and that protein is stuck hard to the ion resin. Maybe denaturing proteins with heat at the end ot the tea steeping step could help (boil at 100C for a few minutes)?
Anyway, will keep on trying but ideas welcome.