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I ran some elution tests on a "mother" resin. This time a 4% NaOH solution was used to adjust the pH during the "batch" elution process.First used water. Looks pH was 8.3, the 4% NaOH moved it up to 9.4 with 3 drops, the pH increase was continuous as far as I could tell and stuff was being eluted based on UV and visible light changes.Next I used everclear (75% alcohol). This was interesting. The pH would shoot up after each drop of NaOH and then drop over the next few minutes. I think this is consistent with eluting something in the formR-XH+ + NaOH -> R-Na+ + X + H2OThree drops were used during the everclear elution, this is what the pH did ( =~ 5 minutes)8.5 -> -> 11.6 -> -> 8.7 -> -> 11.7 -> -> 8.9 -> -> 11.9 -> -> 9.4This elute was pulled from the resin and acidified to pH6.5 with a dash of vitaminC. HPBCD was also present to try to protect from oxydation.Will be testing this process further, doing bioassays and reporting back.
I ran some elution tests on a "mother" resin. This time a 4% NaOH solution was used to adjust the pH during the "batch" elution process.
First used water. Looks pH was 8.3, the 4% NaOH moved it up to 9.4 with 3 drops, the pH increase was continuous as far as I could tell and stuff was being eluted based on UV and visible light changes.
Next I used everclear (75% alcohol). This was interesting. The pH would shoot up after each drop of NaOH and then drop over the next few minutes. I think this is consistent with eluting something in the form
R-XH+ + NaOH -> R-Na+ + X + H2O
Three drops were used during the everclear elution, this is what the pH did ( =~ 5 minutes)
8.5 -> -> 11.6 -> -> 8.7 -> -> 11.7 -> -> 8.9 -> -> 11.9 -> -> 9.4
This elute was pulled from the resin and acidified to pH6.5 with a dash of vitaminC. HPBCD was also present to try to protect from oxydation.
Will be testing this process further, doing bioassays and reporting back.