• Members of the previous forum can retrieve their temporary password here, (login and check your PM).

Reply to thread

I ran some elution tests on a "mother" resin. This time a 4% NaOH solution was used to adjust the pH during the "batch" elution process.


First used water. Looks pH was 8.3, the 4% NaOH moved it up to 9.4 with 3 drops, the pH increase was continuous as far as I could tell and stuff was being eluted based on UV and visible light changes.


Next I used everclear (75% alcohol). This was interesting. The pH would shoot up after each drop of NaOH and then drop over the next few minutes. I think this is consistent with eluting something in the form


R-XH+ + NaOH -> R-Na+ + X + H2O


Three drops were used during the everclear elution, this is what the pH did (⌚ =~ 5 minutes)


8.5 -> 💧 -> 11.6 -> ⌚ -> 8.7 -> 💧 -> 11.7 -> ⌚  -> 8.9 -> 💧 -> 11.9 -> ⌚ -> 9.4


This elute was pulled from the resin and acidified to pH6.5 with a dash of vitaminC. HPBCD was also present to try to protect from oxydation.


Will be testing this process further, doing bioassays and reporting back.


Back
Top Bottom