Innocent
Rising Star
Experiment as follows:
1. A single mashed HBWR seed in a test tube
2. Added distilled water
3. Acidification to pH 3 with citric acid (LSA water soluble in acidic conditions)
4. Added naphtha (defat)
5. Shake
6. Remove naphtha layer
7. Under UV light the water glows blue
8. Added sodium carbonate to pH 9
9. Added xylene and shake
10. Notice something white climbing up the glass walls from water to xylene layer
11. Under UV light water layer glows green. Xylene layer either did not glow or glows at same colour as UV light.
12. Added citric acid to pH 3
13. Under UV light water glows blue again
14. Xylene layer still not glowing
UV wavelength was 375nm
What chemical is responsible for the florescent, pH dependant wavelength behavior?
Initially thought it was LSA but if this was the case why does the water solution glow in basic conditions and the xylene doesn't?
Has this been previously investigated? If so haven't found anything on it as of yet.
1. A single mashed HBWR seed in a test tube
2. Added distilled water
3. Acidification to pH 3 with citric acid (LSA water soluble in acidic conditions)
4. Added naphtha (defat)
5. Shake
6. Remove naphtha layer
7. Under UV light the water glows blue
8. Added sodium carbonate to pH 9
9. Added xylene and shake
10. Notice something white climbing up the glass walls from water to xylene layer
11. Under UV light water layer glows green. Xylene layer either did not glow or glows at same colour as UV light.
12. Added citric acid to pH 3
13. Under UV light water glows blue again
14. Xylene layer still not glowing
UV wavelength was 375nm
What chemical is responsible for the florescent, pH dependant wavelength behavior?
Initially thought it was LSA but if this was the case why does the water solution glow in basic conditions and the xylene doesn't?
Has this been previously investigated? If so haven't found anything on it as of yet.