this is the first time the details of a 1996 p. inacarnata + acacia extract huasca experiment have been released outside of the ethnobotanic research community: (an incarnata vaporized extract experiment is referred to in Voogelbreinder 2009)
"...approx. 1kg of purchased dry passiflora incarnata stem & leaf was boiled X3 in rain water...the large amount of liquid was combined and reduced over several hours, turning increasingly dark purple/brown and thick..,while still possible it was filtered several times through fine cheesecloth...a point of viscosity was reached at which 1 hypothesized dose (333grams of herb) equalled a large glass or coffee mug of thick liquid (the aromas during the process were noted to be somewhat mildly psychoactive)
...A dosage of estimated at = 400 grams herb was consumed by two subjects...5 minutes afterwards approx. 50-70mg of a. obtusifolia extract as the freebase was rapidly swallowed and chased with water/lemon juice (to remove the awful freebase aftertaste)
...Within 10 minutes strong sedative and 'anti-depressant' effects from the passiflora were felt...as these grew (without yet tryptamine effects) it was commented that this plant alone was a satisfying experience...A shiny and pleasant 'haze' surrounded lights...At around 90 minutes, after it was pondered that maybe MAO inhibition was not sufficient, tryptamine-like effects (geometries, '4D-ness', auditory) rapidly grew over 10 minutes accompanied by sudden purging...
...Both subjects found the intensity of colors beyond their previous experiments with a. extract and p. harmala or b. caapi...'fantastic bright blues, reds, purples and golden hues.'...It was subjectively judged as more sedative or narcotic than rue or caapi with a strong 'dreaminess'...rue was judged the 'clearest', caapi unique in other ways...
'It made the tryptamines very dreamy, colorful, and beautiful...very feminine.'
...After a linear 'plateau' of 30 minutes, effects gradually subsided over 2-4 hours, with a sense of sedation up to 5hrs afterwards. Overall, it was very positive...'It lacked the punch of rue, but had such a lovely dreamy quality...very sensual...'..."
it is possible some of the MAOI activity was due to the flavonoids Apigenin and Kaempferol, as well as the ß-carbolines harmine, harmaline, harman, norharman and harmol (present at 0.1-0.2% [Hultin]; Gracie & Zarkov claim up to 0.5%)
Flavinoids are present at around 1.5%,or more, in stems and leaves..regarding Kaempferol, also found in Ginkgo biloba, from:
B. D. Sloley et al.
["Identification of Kaempferol as a Monoamine Oxidase Inhibitor and Potential Neuroprotectant in Extracts of Ginkgo Biloba Leaves" Journal of Pharmacy and Pharmacology Volume 52, Issue 4, pages 451–459, April 2000]
Ginkgo biloba leaf extract was shown to produce in-vitro inhibition of rat brain MAO-A and -B. The Ginkgo biloba extract was chromatographed on a reverse-phase HPLC system and two of the components isolated were shown to be MAO inhibitors (MAOIs). These MAOIs were identified by high-resolution mass spectrometry as kaempferol and isorhamnetin. Pure kaempferol and a number of related flavonoids were examined as MAOIs in-vitro. Kaempferol, apigenin and chrysin proved to be potent MAOIs, but produced more pronounced inhibition of MAO-A than MAO-B. IC50 (50% inhibition concentration) values for the ability of these three flavones to inhibit MAO-A were 7 times 10−7, 1 times 10−6 and 2 times 10−6m, respectively.
some potent MAOI flavonoids include quercitrin, isoquercitrin, rutin, and quercetin, which were isolated from the leaves of Melastoma candidum. They exhibited an inhibitory effect on MAO-B. The IC50 values 19.06, 11.64, 3.89, and 10.89 μM respectively/
["Monoamine Oxidase B and Free Radical Scavenging Activities of Natural Flavonoids in Melastoma candidum "
Mei-Hsien Lee et al. J. Agric. Food Chem., 2001, 49 (11), pp 5551–5555]
..many more plants than previously thought could be effective MAO inhibitors..
i have attached a cool paper on flavonoids and their bio-activity
"Flavonoids and the CNS" Anna K. Jäger and Lasse Saaby Molecules 2011, 16, 1471-1485
en.wikipedia.org
