I am thinking of this tincture for my clients with PTSD, depression, and/or anxiety. Thoughts?

Amanita Muscaria Microdose Tincture

One Dose = 500mg dried mushroom = 20mg of 25:1 extract.

10mL tinctures = 200 drops = 5 drops per dose is 40 doses.

800mg of 25:1 extract per tincture.

Add 400mg of l-ascorbic acid (vitamin C) as a preservative.

To make up a 10mL tincture

- 9mL vodka

- 800mg 25:1 extract

- 400mg l-ascorbic acid

Contains isoxazole alkaloids

- ibotenic acid

- muscimol

- muscazone

- muscaridine

Ibotenic acid is a neurotoxin. UV light decarboxylates ibotenic acid to muscimol (the safe alkaloid). However, UV light also degrades muscimol into an inactive compound.

After three days of sun drying there is 47% ibotenic acid left with maximum muscimol content.

After eleven days of sun drying there is 8% ibotenic acid left but only 32% of muscimol left.

Decarboxylation is a sigmoidal trend, so between 3 and 11 days the decarboxylation will be approximately linear. Each day between 3 and 11 days will decarboxylate 4.875% ibotenic acid and 8.5% muscimol. If we use 7 days drying we will have 27.5% ibotenic acid and 66% muscimol left.

At 3 days sunlight a gram has 582μg ibotenic acid and 256μg muscimol.

At 11 days sunlight a gram has 130μg ibotenic acid and 119μg muscimol.

At 7 days sunlight a gram has 356μg ibotenic acid and 188μg muscimol.

The 7 day sunlight 500mg dried dose (20mg 25:1 extract) contains 176μg ibotenic acid and 144μg muscimol, making this a microdose.

- Toxic hallucinations dose for ibotenic acid is 30-60mg.

- Hallucination dose for muscimol is 10-15mg.

7 day sunlight exposed 1.4-2.1g 25:1 extract contains 10-15mg of muscimol but only 12-28mg ibotenic acid.

This makes 7 days sunlight a perfect amount.

References Used

1. https://www.jstage.jst.go.jp/article/shokueishi1960/34/2/34_2_153/_pdf/-char/en

2. Coma in the course of severe poisoning after consumption of red fly agaric (Amanita muscaria).                             | Acta Biochimica Polonica