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So my rough plan with all of these is:

With the cubensis colonise the barley grain fully, maybe do a grain to grain transfer to expand it more, either way spawning onto a coir bulk substrate in a nice big tub.

When the liquid culture is growing well I'll try and colonise some more barley grain too.

With the oysters I want to try making a big bag of dowel spawn to inoculate logs with, although some grain will also be kept for use as grain spawn onto straw.

With the turkey tail I'll also make some dowel spawn but I could also try propagating it onto woodchip directly.


Barley grain jar details:

Just big pickle jars, two holes were made in the top. One was cut with a hole punch and self-sealing injection ports inserted and crimped around. The other was a bigger hole for air exchange.

Under each lid there's a filter disc and just to ensure sterility some micropore was taped over the large hole.

The bag was an 'oven bag' with a round hole cut in it with a filter taped over and for injection just a layer of micropore on each side of the plastic, taped over after use.

The barley was cooked until 'al dente' and drained very well and packed into jars.

All of the above sterilised for 60 minutes @ 10 psi in a large 15L (3 gallon) pressure cooker.

Inoculation with spore syringes, approx 1ml into each jar. I didn't inoculate in sterile conditions just in a clean kitchen and swabbing injection points and needle between each use, seems to have worked fine.


Any questions please ask!


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