Small batch extraction for testing

[cyantific]

If one wanted to have a portion of their cacti collection tested, what would the best way to go about doing this? For example, Out of 100 plants they want to test 20 of them. What would be the quickest tek to generate the minimum amount needed for analytical testing? (on 20 specimens)

 

[oetzi13]

collect small amounts(5g dry is enough)of each plant and use the Cielo tek

 

[cyantific]

cool, thank you. If you had to guess, how much time would it take to perform each of the 5g. extractions? Any idea how much material is required for testing?

 

[Cheelin]

If one wanted to have a portion of their cacti collection tested, what would the best way to go about doing this? For example, Out of 100 plants they want to test 20 of them. What would be the quickest tek to generate the minimum amount needed for analytical testing? (on 20 specimens)

I assume you are interested in testing for % M content.

Short of extraction, I know of no way to do this at home.

A very doable tek (quick & easy one, once you learn it), is Loveall’s Cielo tek: CIELO - DMT-Nexus Wiki
The “Ethyl Acetate Approach” thread referenced in the wiki, provides detailed info, including my initial work developing a small-scale version for determining plant M % yield. So far, I can reliably determine % yield from as little as 5g of cactus powder. I am working to miniaturize this to 1g samples using a testube. The tek has the potential to be reliably used as a titrimetric approach, but I don’t have the skillset to take it that far.

 

[Loveall]

collect small amounts(5g dry is enough)of each plant and use the Cielo tek

Just want to add that Cheelin has posted details on small scale extractions in the CIELO Tek thread.

 

[Cheelin]

cool, thank you. If you had to guess, how much time would it take to perform each of the 5g. extractions? Any idea how much material is required for testing?

Tek takes an hour of processing time. Elapsed time is upto 72 hours, depending upon crystallization option chosen, potentially this can be reduced to 4 hrs, though i haven’t yet tested the fast crystallization method at this sample size.

Assuming 95% wt lost to get the material sufficiently dry, you’d need 100g fresh material to provide 5g dry.

 

[cyantific]

I assume you are interested in testing for % M content.
Short of extraction, I know of no way to do this at home.

mini extractions with results sent to a lab such as Solaris.

Just want to add that Cheelin has posted details on small scale extractions in the CIELO Tek thread.

I'll check that out thanks!

 

[Cheelin]

I assume you are interested in testing for % M content.
Short of extraction, I know of no way to do this at home.

Correct, mini extractions with results sent to a lab such as Solaris.

As i understand it, labs will tell you purity level, from a miniscule sample. But, if they offer % yield analysis, the sample size would be much larger.

 

[cyantific]

As i understand it, labs will tell you purity level, from a miniscule sample. But, if they offer % yield analysis, the sample size would be much larger.

This type of report below was shared by urica in the Cactus Analysis Thread. It was performed by the lab Energy Control. Just not sure how much extract was submitted for analysis.

ENERGY CONTROL RESULTS: 88% of this extract was mescaline, so 0.0385% fresh, 0.76% presumed dry. 385 mgs mescaline per kg fresh plant.

 

[Cheelin]

As i understand it, labs will tell you purity level, from a miniscule sample. But, if they offer % yield analysis, the sample size would be much larger.

This type of report below was shared by urica in the Cactus Analysis Thread. It was performed by the lab Energy Control. Just not sure how much extract was submitted for analysis.

ENERGY CONTROL RESULTS: 88% of this extract was mescaline, so 0.0385% fresh, 0.76% presumed dry. 385 mgs mescaline per kg fresh plant.

Look at the cielo wiki link above, it has a Solaris purity analysis.

 

[Cheelin]

Here is a link to a post in the ethyl acetate update thread that shows one of my cielo trials on 5g cactus powder samples (same powder w diff treatments): Ethyl acetate approach [CIELO] - Preparation - Welcome to the DMT-Nexus

 

[Cheelin]

BTW, although I haven’t tested this, i assume reliable product purity determination is a characteristic of the chosen extraction method; reliable % yield determination of product at that purity level is a characteristic of the source material.

If so, a single purity analysis per properly conducted extraction method would be sufficient; while yield % would need to be determined for each different source material.