Hey folks,
I have prepared an imaginary study to show how one could theoretically solve the mystery of jungle spice. Here is the report. I will use SWIM to describe the imaginary person wrote the report.
SWIM prepared a crude jungle spice extract by adding powdered MH root bark into pH 12.3 KOH solution. Let sit on 50C for about an hour. extracted two times with toluene. evaporated toluene under reduced pressure. left with some residue that smelled like spice and had a brown/orange residue.
SWIM redissolved this residue in 90% acetonitrile and 10% water solution (for LC-MS analysis these solvents were used). SWIM then ran LC-MS using water acetonitrile (0.1% formic acid) gradient. The MS had an AP-ESI probe (which are useful for finding molecular ions with limited fragmentation).
Anyway the UV (254nm) chromatogram there was one large large tailing peak which ionized well and a couple of minor peaks that gave no ions in the MS. Tailing means the peak was a bad shape and next time SWIM needs to use stronger acid in mobile phase to get a better shape but anyway. SWIM looked at the total ion count of the large peak.
The peak had the following ions: 491 then 477 (molecular mass of yuremamine + 1 hydrogen) then the base peak at 189 (dmt) and another fragment at 115. There are more ions which confirm the structure which SWIM needs to work out the fragments still but anyway we have yuremamine present SWIM is nearly convinced. But how to explain the 491 peak? 491-477 is 14. SWIM thinks this peak may be some kind of adduct forming in the instrument but what typically gives a + 14 peak? SWIM is looking into this more.
Anyway so it seems yuremamine is present in jungle spice. more structural work to come. also the yuremamine peak could be overlapping with a dmt peak too but SWIM needs to improve the chromatography to solve that problem. SWIM is also preparing yuremamine extract the same way as Vepsalainen et al 2005 to confirm results as well.
so then what happens when you smoke it? SWIM analyzed the same material by GC-MS which at the injector is 280 degrees C. enough to vaporize dmt. anyway all SWIM sees is one peak for dmt and another peak for a beta carboline (the same one ID'd before) about ten times smaller then the dmt peak and another peak which is a plastisizer (SWIM used plastic in filtering the sample with solvent so plastisizer was present this almost always happens with plastics) and some kind of phenolic compound (which makes sense if yuremamine degraded).
SWIM will get more details out in the near future but SWIM thinks that what happens when you make STB jungle spice extracts is that you are extracting yuremamine and dmt (ratios probably vary depending on how extract was done). when you smoke it you convert most of the yuremamine into dmt and some of it into a beta carboline and some phenolics.
So anyway thats how you solve the mystery of jungle spice. I hope the report is useful. I am a bit messy now but can answer any questions or provide more fake data soon.
I have prepared an imaginary study to show how one could theoretically solve the mystery of jungle spice. Here is the report. I will use SWIM to describe the imaginary person wrote the report.
SWIM prepared a crude jungle spice extract by adding powdered MH root bark into pH 12.3 KOH solution. Let sit on 50C for about an hour. extracted two times with toluene. evaporated toluene under reduced pressure. left with some residue that smelled like spice and had a brown/orange residue.
SWIM redissolved this residue in 90% acetonitrile and 10% water solution (for LC-MS analysis these solvents were used). SWIM then ran LC-MS using water acetonitrile (0.1% formic acid) gradient. The MS had an AP-ESI probe (which are useful for finding molecular ions with limited fragmentation).
Anyway the UV (254nm) chromatogram there was one large large tailing peak which ionized well and a couple of minor peaks that gave no ions in the MS. Tailing means the peak was a bad shape and next time SWIM needs to use stronger acid in mobile phase to get a better shape but anyway. SWIM looked at the total ion count of the large peak.
The peak had the following ions: 491 then 477 (molecular mass of yuremamine + 1 hydrogen) then the base peak at 189 (dmt) and another fragment at 115. There are more ions which confirm the structure which SWIM needs to work out the fragments still but anyway we have yuremamine present SWIM is nearly convinced. But how to explain the 491 peak? 491-477 is 14. SWIM thinks this peak may be some kind of adduct forming in the instrument but what typically gives a + 14 peak? SWIM is looking into this more.
Anyway so it seems yuremamine is present in jungle spice. more structural work to come. also the yuremamine peak could be overlapping with a dmt peak too but SWIM needs to improve the chromatography to solve that problem. SWIM is also preparing yuremamine extract the same way as Vepsalainen et al 2005 to confirm results as well.
so then what happens when you smoke it? SWIM analyzed the same material by GC-MS which at the injector is 280 degrees C. enough to vaporize dmt. anyway all SWIM sees is one peak for dmt and another peak for a beta carboline (the same one ID'd before) about ten times smaller then the dmt peak and another peak which is a plastisizer (SWIM used plastic in filtering the sample with solvent so plastisizer was present this almost always happens with plastics) and some kind of phenolic compound (which makes sense if yuremamine degraded).
SWIM will get more details out in the near future but SWIM thinks that what happens when you make STB jungle spice extracts is that you are extracting yuremamine and dmt (ratios probably vary depending on how extract was done). when you smoke it you convert most of the yuremamine into dmt and some of it into a beta carboline and some phenolics.
So anyway thats how you solve the mystery of jungle spice. I hope the report is useful. I am a bit messy now but can answer any questions or provide more fake data soon.
