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Excellent work Trickster. I have switched my focus to sonication. I purchased a converter/horn and a 750 watt power supply for a very good price, but they were mismatched components.. After fooling around with it for a couple weeks to make the mismatched converter work with the supply I am able to get very high energy delivery to my samples.The point is in this process I too have discovered with the help of Benzyme that elegance of not over acidifying my extractions. Using just 3 ml of 8% vinegar in ~500 ml of water to process 100 grams of MHRB I sonicted for about 1 hour (1/2 hour per 250 ml), I did gross filtering and decanting, and then basified to about pH = 13 using 5 grams of NaOH. It is good to conserve chemicals.. I could have used a lot less lye. I got a modest yield of about 0.6% using Pentane/Hexane PE doing several (8) pulls in my separation funnel. The result was my usual snow white powder.
Excellent work Trickster. I have switched my focus to sonication. I purchased a converter/horn and a 750 watt power supply for a very good price, but they were mismatched components.. After fooling around with it for a couple weeks to make the mismatched converter work with the supply I am able to get very high energy delivery to my samples.
The point is in this process I too have discovered with the help of Benzyme that elegance of not over acidifying my extractions. Using just 3 ml of 8% vinegar in ~500 ml of water to process 100 grams of MHRB I sonicted for about 1 hour (1/2 hour per 250 ml), I did gross filtering and decanting, and then basified to about pH = 13 using 5 grams of NaOH. It is good to conserve chemicals.. I could have used a lot less lye. I got a modest yield of about 0.6% using Pentane/Hexane PE doing several (8) pulls in my separation funnel. The result was my usual snow white powder.