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Nen wrote ,

But what i would mainly like to postulate in this intro. is the question i used to ask those who argued that
DMT, if purified, was the same whether it was synthetic or extracted from a plant. I would ask them:
"If i shredded up your
grandmother, extracted the DMT out of her cerebralspinal fluid., purified it and asked you to smoke it, would that be the same?"

i realy just loved the way you put it.!
very good analogy!
well said.,

yes , mimosa could easily become a real pest like syrian rue once was in southaustralia,
and still is in some places.
check out AVH africa rue.
found some in sa a few years ago.
brwed up the roots.
was sic brew,

An efficient in vitro production of biomass hairy root cultures and elicitation of β-carboline alkaloids of Peganum harmala was established from P. harmala seedlings. Hairy root culture was arised by inoculation of the excised seedling with Agrobacterium rhizogenes. Analysis of rolC and virC genes by using polymerase chain reaction (PCR) has confirmed the transformation of the root culture. Hairy root cultures were employed to study the production of harmine as the main β-carboline alkaloids. The transformed cultures were incubated with potential elicitors, such as hydrogen peroxides (H2O2), or biosynthetic precursor as tryptophane in order to stimulate the biosynthesis of β-carboline alkaloids, mainly harmine as the major one. Profile and amounts of β-carboline alkaloids were analyzed using capillary GLC–MS.

The results revealed that, treatment of the cultures with H2O2 (0.01%) induced the accumulation of harmine, as the major β-carboline alkaloids in root, by approximately sixfold as compared to the untreated control. The highest stimulation resulted in a harmine level of ca. 4.5 mg/g DW after 24 h of incubation. Concerning the biosynthetic precursor, tryptophane (2 mM) enhanced the alkaloid production approximately fivefold (0.25–0.3 mg/g DW) within 24 h.

<blockquote data-quote="acacaya" data-source="post: 3810948" data-attributes="member: 36300">Nen wrote , But what i would mainly like to postulate in this intro. is the question i used to ask those who argued thatDMT, if purified, was the same whether it was synthetic or extracted from a plant. I would ask them:&quot;If i shredded up yourgrandmother, extracted the DMT out of her cerebralspinal fluid., purified it and asked you to smoke it, would that be the same?&quot;i realy just loved the way you put it.!very good analogy!well said.,yes , mimosa could easily become a real pest like syrian rue once was in southaustralia,and still is in some places.check out AVH africa rue.found some in sa a few years ago.brwed up the roots.was sic brew,An efficient in vitro production of biomass hairy root cultures and elicitation of β-carboline alkaloids of Peganum harmala was established from P. harmala seedlings. Hairy root culture was arised by inoculation of the excised seedling with Agrobacterium rhizogenes. Analysis of rolC and virC genes by using polymerase chain reaction (PCR) has confirmed the transformation of the root culture. Hairy root cultures were employed to study the production of harmine as the main β-carboline alkaloids. The transformed cultures were incubated with potential elicitors, such as hydrogen peroxides (H2O2), or biosynthetic precursor as tryptophane in order to stimulate the biosynthesis of β-carboline alkaloids, mainly harmine as the major one. Profile and amounts of β-carboline alkaloids were analyzed using capillary GLC–MS.The results revealed that, treatment of the cultures with H2O2 (0.01%) induced the accumulation of harmine, as the major β-carboline alkaloids in root, by approximately sixfold as compared to the untreated control. The highest stimulation resulted in a harmine level of ca. 4.5 mg/g DW after 24 h of incubation. Concerning the biosynthetic precursor, tryptophane (2 mM) enhanced the alkaloid production approximately fivefold (0.25–0.3 mg/g DW) within 24 h.</blockquote>

[QUOTE="acacaya, post: 3810948, member: 36300"] Nen wrote , But what i would mainly like to postulate in this intro. is the question i used to ask those who argued that DMT, if purified, was the same whether it was synthetic or extracted from a plant. I would ask them: "If i shredded up your grandmother, extracted the DMT out of her cerebralspinal fluid., purified it and asked you to smoke it, would that be the same?" i realy just loved the way you put it.! very good analogy! well said., yes , mimosa could easily become a real pest like syrian rue once was in southaustralia, and still is in some places. check out AVH africa rue. found some in sa a few years ago. brwed up the roots. was sic brew, An efficient in vitro production of biomass hairy root cultures and elicitation of β-carboline alkaloids of Peganum harmala was established from P. harmala seedlings. Hairy root culture was arised by inoculation of the excised seedling with Agrobacterium rhizogenes. Analysis of rolC and virC genes by using polymerase chain reaction (PCR) has confirmed the transformation of the root culture. Hairy root cultures were employed to study the production of harmine as the main β-carboline alkaloids. The transformed cultures were incubated with potential elicitors, such as hydrogen peroxides (H2O2), or biosynthetic precursor as tryptophane in order to stimulate the biosynthesis of β-carboline alkaloids, mainly harmine as the major one. Profile and amounts of β-carboline alkaloids were analyzed using capillary GLC–MS. The results revealed that, treatment of the cultures with H2O2 (0.01%) induced the accumulation of harmine, as the major β-carboline alkaloids in root, by approximately sixfold as compared to the untreated control. The highest stimulation resulted in a harmine level of ca. 4.5 mg/g DW after 24 h of incubation. Concerning the biosynthetic precursor, tryptophane (2 mM) enhanced the alkaloid production approximately fivefold (0.25–0.3 mg/g DW) within 24 h. [/QUOTE]