edwardbernays
Esteemed member
My sterile technique is alright. This will be my first time working with agar. Any tips for good equipment to have? Here's my current list:
* reusable autoclavable dishes
* agar
* light malt
* inoculation loop
* 70% IPA
* pressure cooker
* still air-box
* gloves
Prints I'm getting:
* B+ (used print)
* McKennai (used print)
* PESA (fresh)
* Redboy (fresh)
* Blue Meanie (fresh but not profesh)
* Orissa India x Rusty Whyte hybrid (fresh but old, from Solipsis! Rare)
Swabs I'm getting:
* Machine Elf
* Storm Troopers
* PF Classic
Current plating goal (skipping many details):
1. Prep 4 spare agar plates (two water agar, two with malt; this is to give me an idea of the contam present in the environment + a skill check on my technique)
2. Prep 2 plates per selected specimen in SAB
Since this is my first time working with agar for mycology, I'm thinking I should select specimens that are easy to reacquire. Are swabs easier to work with than prints for this? Also, any tips for constructing an SAB, or good agar emoluments for fighting off contam?
* reusable autoclavable dishes
* agar
* light malt
* inoculation loop
* 70% IPA
* pressure cooker
* still air-box
* gloves
Prints I'm getting:
* B+ (used print)
* McKennai (used print)
* PESA (fresh)
* Redboy (fresh)
* Blue Meanie (fresh but not profesh)
* Orissa India x Rusty Whyte hybrid (fresh but old, from Solipsis! Rare)
Swabs I'm getting:
* Machine Elf
* Storm Troopers
* PF Classic
Current plating goal (skipping many details):
1. Prep 4 spare agar plates (two water agar, two with malt; this is to give me an idea of the contam present in the environment + a skill check on my technique)
2. Prep 2 plates per selected specimen in SAB
Since this is my first time working with agar for mycology, I'm thinking I should select specimens that are easy to reacquire. Are swabs easier to work with than prints for this? Also, any tips for constructing an SAB, or good agar emoluments for fighting off contam?