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ADDITIONAL PREPARATION (2)

The above mentioned 543mg of A. floribunda were cut with scissors and soaked in one of the "big" test tubes that comes with the reagent bottles. The test tube was filled about 1/3 of its height. The preparation was left to soak for about 10 hours. The liquid was combined with the remaining liquid from the A. floribunda preparation that was tested above.

The liquid was left to evaporate and the remaining oil was transferred to a small test tube. The volume of the oil was approximately equivalent to the volume of 2-3 match heads. That test tube was filled only to 1mm below the end of the conical bottom section. It was shaken several times over a period of 30 min to allow the extracted oil to dissolve completely.

The A. floribunda liquid was transferred to Columns A and C of "Plate F". Transfer per column was repeated 8x.
The middle Column B received 2 transfers of the ACRB extract that was used above as reference.

Two more Plates "I" and "II" were prepared just using the ACRB extract for two objectives:
1) Provide a reference result for more reagents.
2) Figure out what the third spot above the DMT spot is.

Each column of Plates "I" and "II" received 2 transfers of the ACRB extract preparation.

FULL KEY

Note: The labelling of the plates was done pretty late in the process, all pictures show the plates in the same order.

*Plate I* (left)

- Column A: ACRB extract - Ehrlich
- Column B: ACRB extract - Liebermann
- Column C: ACRB extract - Hofmann

*Plate II* (middle)

- Column A: ACRB extract - Mandelin
- Column B: ACRB extract - Froehde
- Column C: ACRB extract - Mecke

*Plate F* (right)

- Column A: A. floribunda - Ehrlich
- Column B: ACRB extract - No reagent
- Column C: A. floribunda - Ehrlich on NMT spot (forgot to switch the reagent), Froehde on all other spots above

Note: Froehde was chosen, because most reagents behaved similar for DMT and NMT. The Froehde reaction on NMT was slower, thus allowing to distinguish between NMT and DMT. The effect is shown in the earlier, non-labelled reagent result picture.

QUESTIONS / DISCUSSION

1) What could the third (top) spot in the ACRB extract be ? Any why ?

2) In the blacklight picture, there seem to be spots in the lower area of the "green plant oil flame", in the A column on the left, in the C column on both sides, but a bit smaller. In the "early" picture, the Ehrlich might show a faint reaction, but it's hard to interpret. The spot is higher than DMT and with Froehde it seems to have turned brown. The spot is somewhat higher than the DMT reference. What could that be? The wikipedia page on Froehde mentions brown for ephedrine and opiates, so this can only be a start.

3) There seems to be a clear "band" at the NMT height.

4) There's the skewed upper "band" which in the early pictures only shows a lighter Ehrlich reaction, but in the several hours late picture, it can be seen a bit more towards the left side of the card and the band seems to stop at the exact height of the DMT reference. So could this upper band be DMT? I guess so, but why is it skewed? I'm thinking transferring the liquid 8x was too much, but can too much material lead to such skewed bands ?

5) There is the two big pinkish areas between the NMT and DMT bands. Approx. the center is right in the middle between DMT and NMT. I was wondering whether that could be also an artifact of using too much material, but it's weird that it stays and extends around that height instead of intensifying the DMT band, so I guess it can't be DMT. Further, it's bit unclear what Froehde on the C column did. It might show a super slight browning on the picture but nothing like on the ACRB DMT reference. With bare eyes, this was not really observable, I first thought it didn't do anything. Inspecting the HDR photo, it shows a slight blueish hued halo, which is also present around the top mystery spots around the "plant oil flame". So, not sure if that's just the Froehde under HDR or if this indicates a reaction of sort.

In total, I think transferring 8x was too much esp. with all the plant oil present, so I'll repeat with less once I'm back home (currently traveling).

However, it would be really great to get feedback on the above points.

<blockquote data-quote="plantatron" data-source="post: 3790815" data-attributes="member: 30845">ADDITIONAL PREPARATION (2)The above mentioned 543mg of A. floribunda were cut with scissors and soaked in one of the &quot;big&quot; test tubes that comes with the reagent bottles. The test tube was filled about 1/3 of its height. The preparation was left to soak for about 10 hours. The liquid was combined with the remaining liquid from the A. floribunda preparation that was tested above.&nbsp; The liquid was left to evaporate and the remaining oil was transferred to a small test tube. The volume of the oil was approximately equivalent to the volume of 2-3 match heads. That test tube was filled only to 1mm below the end of the conical bottom section. It was shaken several times over a period of 30 min to allow the extracted oil to dissolve completely.The A. floribunda liquid was transferred to Columns A and C of &quot;Plate F&quot;. Transfer per column was repeated 8x.The middle Column B received 2 transfers of the ACRB extract that was used above as reference.Two more Plates &quot;I&quot; and &quot;II&quot; were prepared just using the ACRB extract for two objectives:1) Provide a reference result for more reagents.2) Figure out what the third spot above the DMT spot is.Each column of Plates &quot;I&quot; and &quot;II&quot; received 2 transfers of the ACRB extract preparation.FULL KEYNote: The labelling of the plates was done pretty late in the process, all pictures show the plates in the same order.*Plate I* (left)- Column A: ACRB extract - Ehrlich- Column B: ACRB extract - Liebermann- Column C: ACRB extract - Hofmann*Plate II* (middle)- Column A: ACRB extract - Mandelin- Column B: ACRB extract - Froehde- Column C: ACRB extract - Mecke*Plate F* (right)- Column A: A. floribunda - Ehrlich- Column B: ACRB extract - No reagent- Column C: A. floribunda - Ehrlich on NMT spot (forgot to switch the reagent), Froehde on all other spots aboveNote: Froehde was chosen, because most reagents behaved similar for DMT and NMT. The Froehde reaction on NMT was slower, thus allowing to distinguish between NMT and DMT. The effect is shown in the earlier, non-labelled reagent result picture.QUESTIONS / DISCUSSION1) What could the third (top) spot in the ACRB extract be ? Any why ?2) In the blacklight picture, there seem to be spots in the lower area of the &quot;green plant oil flame&quot;, in the A column on the left, in the C column on both sides, but a bit smaller. In the &quot;early&quot; picture, the Ehrlich might show a faint reaction, but it&#039;s hard to interpret. The spot is higher than DMT and with Froehde it seems to have turned brown. The spot is somewhat higher than the DMT reference. What could that be? The wikipedia page on Froehde mentions brown for ephedrine and opiates, so this can only be a start.3) There seems to be a clear &quot;band&quot; at the NMT height.4) There&#039;s the skewed upper &quot;band&quot; which in the early pictures only shows a lighter Ehrlich reaction, but in the several hours late picture, it can be seen a bit more towards the left side of the card and the band seems to stop at the exact height of the DMT reference. So could this upper band be DMT? I guess so, but why is it skewed? I&#039;m thinking transferring the liquid 8x was too much, but can too much material lead to such skewed bands ?5) There is the two big pinkish areas between the NMT and DMT bands. Approx. the center is right in the middle between DMT and NMT. I was wondering whether that could be also an artifact of using too much material, but it&#039;s weird that it stays and extends around that height instead of intensifying the DMT band, so I guess it can&#039;t be DMT. Further, it&#039;s bit unclear what Froehde on the C column did. It might show a super slight browning on the picture but nothing like on the ACRB DMT reference. With bare eyes, this was not really observable, I first thought it didn&#039;t do anything. Inspecting the HDR photo, it shows a slight blueish hued halo, which is also present around the top mystery spots around the &quot;plant oil flame&quot;. So, not sure if that&#039;s just the Froehde under HDR or if this indicates a reaction of sort.In total, I think transferring 8x was too much esp. with all the plant oil present, so I&#039;ll repeat with less once I&#039;m back home (currently traveling).However, it would be really great to get feedback on the above points.</blockquote>

[QUOTE="plantatron, post: 3790815, member: 30845"] ADDITIONAL PREPARATION (2) The above mentioned 543mg of A. floribunda were cut with scissors and soaked in one of the "big" test tubes that comes with the reagent bottles. The test tube was filled about 1/3 of its height. The preparation was left to soak for about 10 hours. The liquid was combined with the remaining liquid from the A. floribunda preparation that was tested above. The liquid was left to evaporate and the remaining oil was transferred to a small test tube. The volume of the oil was approximately equivalent to the volume of 2-3 match heads. That test tube was filled only to 1mm below the end of the conical bottom section. It was shaken several times over a period of 30 min to allow the extracted oil to dissolve completely. The A. floribunda liquid was transferred to Columns A and C of "Plate F". Transfer per column was repeated 8x. The middle Column B received 2 transfers of the ACRB extract that was used above as reference. Two more Plates "I" and "II" were prepared just using the ACRB extract for two objectives: 1) Provide a reference result for more reagents. 2) Figure out what the third spot above the DMT spot is. Each column of Plates "I" and "II" received 2 transfers of the ACRB extract preparation. FULL KEY Note: The labelling of the plates was done pretty late in the process, all pictures show the plates in the same order. *Plate I* (left) - Column A: ACRB extract - Ehrlich - Column B: ACRB extract - Liebermann - Column C: ACRB extract - Hofmann *Plate II* (middle) - Column A: ACRB extract - Mandelin - Column B: ACRB extract - Froehde - Column C: ACRB extract - Mecke *Plate F* (right) - Column A: A. floribunda - Ehrlich - Column B: ACRB extract - No reagent - Column C: A. floribunda - Ehrlich on NMT spot (forgot to switch the reagent), Froehde on all other spots above Note: Froehde was chosen, because most reagents behaved similar for DMT and NMT. The Froehde reaction on NMT was slower, thus allowing to distinguish between NMT and DMT. The effect is shown in the earlier, non-labelled reagent result picture. QUESTIONS / DISCUSSION 1) What could the third (top) spot in the ACRB extract be ? Any why ? 2) In the blacklight picture, there seem to be spots in the lower area of the "green plant oil flame", in the A column on the left, in the C column on both sides, but a bit smaller. In the "early" picture, the Ehrlich might show a faint reaction, but it's hard to interpret. The spot is higher than DMT and with Froehde it seems to have turned brown. The spot is somewhat higher than the DMT reference. What could that be? The wikipedia page on Froehde mentions brown for ephedrine and opiates, so this can only be a start. 3) There seems to be a clear "band" at the NMT height. 4) There's the skewed upper "band" which in the early pictures only shows a lighter Ehrlich reaction, but in the several hours late picture, it can be seen a bit more towards the left side of the card and the band seems to stop at the exact height of the DMT reference. So could this upper band be DMT? I guess so, but why is it skewed? I'm thinking transferring the liquid 8x was too much, but can too much material lead to such skewed bands ? 5) There is the two big pinkish areas between the NMT and DMT bands. Approx. the center is right in the middle between DMT and NMT. I was wondering whether that could be also an artifact of using too much material, but it's weird that it stays and extends around that height instead of intensifying the DMT band, so I guess it can't be DMT. Further, it's bit unclear what Froehde on the C column did. It might show a super slight browning on the picture but nothing like on the ACRB DMT reference. With bare eyes, this was not really observable, I first thought it didn't do anything. Inspecting the HDR photo, it shows a slight blueish hued halo, which is also present around the top mystery spots around the "plant oil flame". So, not sure if that's just the Froehde under HDR or if this indicates a reaction of sort. In total, I think transferring 8x was too much esp. with all the plant oil present, so I'll repeat with less once I'm back home (currently traveling). However, it would be really great to get feedback on the above points. [/QUOTE]