Elrik
Rising Star
I observed an unexpected phenomena that I havent ever seen explicitly mentioned so I figured I'd briefly share.
While doing microscale experiments on harm[al]ine purification I got impurities to precipitate when they really shouldn't have. It got my attention enough to try a dedicated test of the effect.
ca. 200mg of impure red harmaline/harmine•HCl was made into 20 ml solution with distilled water, filtered, centrifuged, and refiltered. It was free of solids. I based it to pH 13+ with a 5ml aliquot of NaOH (aq) and centrifuged. The liquid was discarded and the dark tan solids were dissolved in 6 ml of 5% acetic acid [a clear excess]. The solution was centrifuged and a quantity of nearly black gunk separated out. The liquid was then mansked to make beautiful light golden yellow crystals I'd be proud to eat.
What I suspect is that a strongly colored alkaloidal impurity that is less soluble in vinegar than harmaline acetate was pushed out of solution when the alkaloid solution was made so concentrated. It looked like it did the job of several manskes.
Has anyone else observed that phenomena?
While doing microscale experiments on harm[al]ine purification I got impurities to precipitate when they really shouldn't have. It got my attention enough to try a dedicated test of the effect.
ca. 200mg of impure red harmaline/harmine•HCl was made into 20 ml solution with distilled water, filtered, centrifuged, and refiltered. It was free of solids. I based it to pH 13+ with a 5ml aliquot of NaOH (aq) and centrifuged. The liquid was discarded and the dark tan solids were dissolved in 6 ml of 5% acetic acid [a clear excess]. The solution was centrifuged and a quantity of nearly black gunk separated out. The liquid was then mansked to make beautiful light golden yellow crystals I'd be proud to eat.
What I suspect is that a strongly colored alkaloidal impurity that is less soluble in vinegar than harmaline acetate was pushed out of solution when the alkaloid solution was made so concentrated. It looked like it did the job of several manskes.
Has anyone else observed that phenomena?