2 x 50g packs of cosmetic mimosa tenuiflora stem bark powder.
- the 100g powder was mixed with 500 ml distilled water, then vinegar was added to ph 3, tested with ph measuring strips,
- the mix was put in a modified 3000 ml fido jar with a modified cap to support a manual vacuum pump;
- the jar was vacuumed to -85 kpa, to eliminate oxygen bubbles trapped in the powder
- the jar was then submersed in a 15L ultrasonic cleaner, and sonicated at 50°C for 2 hours
- the acidic solvent was filtered first through a 100 micron mesh filter, then again through 20~30 micron paper coffee filter, to eliminate most of the particulates
- Naoh sollution was added to ph 14, liquid turning dark brown, almost black, and blended
- 100 ml extraction benzin was poured onto the warm mix(40°C), and vigurously agitated, no emulsions formed due to filtering of the solid particulates.
- solvent color turned yellowish, and was recovered with a syringe, and poured onto a rectangular glass tray with a cap.
- glass tray was put in the freezer at -20° for 12 hours
total yield of snow white crystal precipitate was 1,1g
the logic conclusion is stem bark has same if not higher concentration of alcaloid than root bark.
next batch will be using more aggressive approach utilising mae/uae combined to test if higher yelds are possible.
uae, does the trick to pull out every mollecule trapped in the organic matrix. should uae be used on root bark, maybe it'll generate higher yelds, or maybe root bark has a different plant matrix that is easyer to pull from.
Logic say the concentration of alcaloid in both stem and bark root is the same, or even higher in the stem, due to the reason the alcaloid is produced in the first place, as deterent to fauna.
root system is already protected by earth substrate to most of the predators, thus it shouldnt concentrate as much detterent as the part that is most exposed. the only difference between the two types of bark is the resilience/density - root system being more porous/soft in nature due to the need to create the osmotic proceses to pull up nutrients and water from the soil, a property that helps in clasic a/b extractions using heat, whereas the stem bark being more resilient due to lignin and suberin matrix that trap the molecules within, needing a more aggressive approach, that is ultrasonic assisted extraction or microwave assisted extraction.
- the 100g powder was mixed with 500 ml distilled water, then vinegar was added to ph 3, tested with ph measuring strips,
- the mix was put in a modified 3000 ml fido jar with a modified cap to support a manual vacuum pump;
- the jar was vacuumed to -85 kpa, to eliminate oxygen bubbles trapped in the powder
- the jar was then submersed in a 15L ultrasonic cleaner, and sonicated at 50°C for 2 hours
- the acidic solvent was filtered first through a 100 micron mesh filter, then again through 20~30 micron paper coffee filter, to eliminate most of the particulates
- Naoh sollution was added to ph 14, liquid turning dark brown, almost black, and blended
- 100 ml extraction benzin was poured onto the warm mix(40°C), and vigurously agitated, no emulsions formed due to filtering of the solid particulates.
- solvent color turned yellowish, and was recovered with a syringe, and poured onto a rectangular glass tray with a cap.
- glass tray was put in the freezer at -20° for 12 hours
total yield of snow white crystal precipitate was 1,1g
the logic conclusion is stem bark has same if not higher concentration of alcaloid than root bark.
next batch will be using more aggressive approach utilising mae/uae combined to test if higher yelds are possible.
uae, does the trick to pull out every mollecule trapped in the organic matrix. should uae be used on root bark, maybe it'll generate higher yelds, or maybe root bark has a different plant matrix that is easyer to pull from.
Logic say the concentration of alcaloid in both stem and bark root is the same, or even higher in the stem, due to the reason the alcaloid is produced in the first place, as deterent to fauna.
root system is already protected by earth substrate to most of the predators, thus it shouldnt concentrate as much detterent as the part that is most exposed. the only difference between the two types of bark is the resilience/density - root system being more porous/soft in nature due to the need to create the osmotic proceses to pull up nutrients and water from the soil, a property that helps in clasic a/b extractions using heat, whereas the stem bark being more resilient due to lignin and suberin matrix that trap the molecules within, needing a more aggressive approach, that is ultrasonic assisted extraction or microwave assisted extraction.
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