I love synchronicity :d The recent discussion of whether Ayahuasca brews should be acidified or not started about one day after I had made my very first non-acidified brew. As resident Aya experts Ms. Minxx and Acolon both expressed the sentiment that acid brews are more potent than non-acid brews, I became very paranoid that I had left a significant amount of alkaloids in the bark that I had just brewed. Luckily I had not thrown out the bark and thus I drafted up a quick experiment to test the efficiency of acid and non-acid brewing, using The Herbal Percolator. Unfortunately, due to the manner in which I realized I was going to do this experiment, I do not have pictures of every little step (but I do have them of the "important" ones) and the brews were not done side by side. However, as I brew my Ayahuasca the same way every time and recorded the steps I had taken between my brewing and Cosmic Lion's Caapi Extraction, I'm fairly confident that this experiment is a fairly good indicator of the difference in efficiency between using acid or not. The experiment was carried out as follows:
The Non-Acid Brew
1. 50 grams of B. Caapi (Ourinhos) were placed in THP and 10L (200ml/g) of non-acidified, boiling tap water was run through it (my tap water has a pH of 6.1)
2. The brew was reduced by steaming over the course of a day and set to the side. At this point I noticed that this brew was darker than any Caapi brew I had made before, even when I had caramelized the brew by boiling too hard.
3. The 50 grams of previously brewed bark was extracted according to the protocols of Cosmic Lion's Caapi Extraction, with the amounts scaled down by 1/5 (I was dealing with 50g Caapi instead of 250g) Here is the dark brew next to the freebase harmalas in solution, Brew is on the left, Harmalas on the right:
and here's the freebase harmalas in solution:
4. The resulting impure harmalas weighed in at 616mg. This extract was a light tan and of uniform consistency/color, similar to the result from multiple Manskes.
The Acid Brew
1. 50 grams of B. Caapi (Ourinhos) were placed in THP and 10L (200ml/g) of boiling tap water (acidified with 1 Tbsp/15ml of 5% vinegar per Liter of water) was run through it. Here's my THP in action:
2. The brew was reduced by steaming over the course of a day and set to the side. The color of this brew was the brilliant gold I have grown familiar to seeing from my Ourinhos brews. Steaming the brew, as you can see, no bubbles whatsoever:
3. The 50 grams of previously brewed bark was extracted according to the protocols of Cosmic Lion's Caapi Extraction, with the amounts scaled down by 1/5 (I was dealing with 50g Caapi instead of 250g). Here's my large scale THP for running more water through at a given time:
Here's the Harmalas in solution, next to the brew with the flash (as in previous pics), Brew is on the left, Harmalas on the right:
and without the flash so that you can see how much less there is:
4. The resulting impure harmalas weighed in at 301mg. This extract was dark and sparkled like a first Manske.
Here are the two vials of Harmalas. As the extract from the non-acidified brew is lighter, I assume it is a cleaner extract, but I have no analytical equipment with which to verify this. Assuming that the lighter alkaloids are cleaner, this would mean that the difference in total alkaloids between the two extracts is actually greater than the 315mg of weight difference suggests.
Conclusion: Acidified water is more efficient than non-acidified water for brewing Ayahuasca using THP. The extraction run on the bark from the non-acidified brew yielded 104.651% more alkaloids of (what I believe to be) higher quality than the acidified brew. This means that the bark from the acidified brew was more thoroughly exhausted than the bark from the non-acidified brew, following the initial brewing.
NOTES: The non-acidified brew was significantly darker than the acidified brew. However, once the already-brewed Caapi was subjected to acidified THP extractions for CL's tek, the initially non-acidified brew was a much deeper yellow-gold than the initially-acidified brew. I can't for the life of me figure out what this is as a result of. Could the acid prevent some darker tannins from being pulled? Does it maybe turn a darker tannin lighter by acidifying? Also, the harmalas yielded from the acidified brew were darker than those extracted from the non-acidified brewed bark. To my mind this means that CL's tek was "scraping the bottom of the barrel" so to speak, and pulled more impurities, as there were less alkaloids to be pulled from that bark compared to the bark that had not been brewed in acidified water.
Heres the non-acidified and acidified brews. Non-acidified on the left, Acidified on the right
The Non-Acid Brew
1. 50 grams of B. Caapi (Ourinhos) were placed in THP and 10L (200ml/g) of non-acidified, boiling tap water was run through it (my tap water has a pH of 6.1)
2. The brew was reduced by steaming over the course of a day and set to the side. At this point I noticed that this brew was darker than any Caapi brew I had made before, even when I had caramelized the brew by boiling too hard.
3. The 50 grams of previously brewed bark was extracted according to the protocols of Cosmic Lion's Caapi Extraction, with the amounts scaled down by 1/5 (I was dealing with 50g Caapi instead of 250g) Here is the dark brew next to the freebase harmalas in solution, Brew is on the left, Harmalas on the right:
![DSCN0564.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0564.jpg%3Ft%3D1280532146&hash=3bd51b55091f4d00f0c84999f664e60a)
and here's the freebase harmalas in solution:
![DSCN0566.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0566.jpg%3Ft%3D1280532152&hash=608d7a0a16fa2aebb5bac632605ef10c)
4. The resulting impure harmalas weighed in at 616mg. This extract was a light tan and of uniform consistency/color, similar to the result from multiple Manskes.
![DSCN0573.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0573.jpg%3Ft%3D1280532134&hash=f071fe0294a20394561dcd6f3bc88e1b)
The Acid Brew
1. 50 grams of B. Caapi (Ourinhos) were placed in THP and 10L (200ml/g) of boiling tap water (acidified with 1 Tbsp/15ml of 5% vinegar per Liter of water) was run through it. Here's my THP in action:
![DSCN0599.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0599.jpg%3Ft%3D1280532123&hash=ca8ba5018f735f3922274c756209fad4)
2. The brew was reduced by steaming over the course of a day and set to the side. The color of this brew was the brilliant gold I have grown familiar to seeing from my Ourinhos brews. Steaming the brew, as you can see, no bubbles whatsoever:
![DSCN0600.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0600.jpg%3Ft%3D1280532116&hash=9a3914275465bac1a108808cd9bbb5bf)
3. The 50 grams of previously brewed bark was extracted according to the protocols of Cosmic Lion's Caapi Extraction, with the amounts scaled down by 1/5 (I was dealing with 50g Caapi instead of 250g). Here's my large scale THP for running more water through at a given time:
![DSCN0602.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0602.jpg%3Ft%3D1280532033&hash=77801539e7b0eee033c85c3fc6caadea)
Here's the Harmalas in solution, next to the brew with the flash (as in previous pics), Brew is on the left, Harmalas on the right:
![DSCN0604.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0604.jpg%3Ft%3D1280531985&hash=67c6d457f14861b4d714576cfb84f0e8)
and without the flash so that you can see how much less there is:
![DSCN0606.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0606.jpg%3Ft%3D1280531958&hash=bcc1d515d4a1a091edaaacda470cdca1)
4. The resulting impure harmalas weighed in at 301mg. This extract was dark and sparkled like a first Manske.
![DSCN0618.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0618.jpg%3Ft%3D1280531775&hash=623ac96874a3663d2864f87f47383892)
Here are the two vials of Harmalas. As the extract from the non-acidified brew is lighter, I assume it is a cleaner extract, but I have no analytical equipment with which to verify this. Assuming that the lighter alkaloids are cleaner, this would mean that the difference in total alkaloids between the two extracts is actually greater than the 315mg of weight difference suggests.
![DSCN0625.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0625.jpg%3Ft%3D1280531721&hash=ef250358c8bebb5d9472076d8b88c03e)
Conclusion: Acidified water is more efficient than non-acidified water for brewing Ayahuasca using THP. The extraction run on the bark from the non-acidified brew yielded 104.651% more alkaloids of (what I believe to be) higher quality than the acidified brew. This means that the bark from the acidified brew was more thoroughly exhausted than the bark from the non-acidified brew, following the initial brewing.
NOTES: The non-acidified brew was significantly darker than the acidified brew. However, once the already-brewed Caapi was subjected to acidified THP extractions for CL's tek, the initially non-acidified brew was a much deeper yellow-gold than the initially-acidified brew. I can't for the life of me figure out what this is as a result of. Could the acid prevent some darker tannins from being pulled? Does it maybe turn a darker tannin lighter by acidifying? Also, the harmalas yielded from the acidified brew were darker than those extracted from the non-acidified brewed bark. To my mind this means that CL's tek was "scraping the bottom of the barrel" so to speak, and pulled more impurities, as there were less alkaloids to be pulled from that bark compared to the bark that had not been brewed in acidified water.
Heres the non-acidified and acidified brews. Non-acidified on the left, Acidified on the right
![DSCN0607.jpg](/proxy.php?image=http%3A%2F%2Fi841.photobucket.com%2Falbums%2Fzz334%2Fthedefaulthoster%2FDSCN0607.jpg%3Ft%3D1280531934&hash=bd02b32546a713fa586b6bf8108923a0)