Can anyone help interpret these HPLC results?

[Samvidbuho]

A while back an analysis was done on two samples, one without re-x and the other with.
Toluene was used as solvent, re-x in hexane.

The first result displays two major peaks. If the main one is N, N - dmt, what would the lesser be? The UV absorption spectra for both is different, but not sure how to read it in order to say what those differences mean..

The second result shows one main peak. If this too is N, N - dmt, why is the absorption spectrum different than the major peak of the first test?

Also included the data from the raw sample before dilutions were known to be necessary. Toluene pulls up so much more than ever would have expected!

 

[endlessness]

Im not at home now to check but i'd bet it's NMT (monomethyl tryptamine). Try to see if you can find the spectra in literature and compare.

 

[downwardsfromzero]

Another possibility might be 2-methyltetrahydro-β-carboline.

What was the source of your analytical sample?

 

[Samvidbuho]

Oh interesting. I can't find anything in the references I have that give the UV spectrum for NMT. I'll keep looking.

Source was mimosa hostilis, dried and powderized.

 

[benzyme]

what are the mobile phases, and what is the stationary phase (the column)?
Retention times can differ from either pH or polarity.

 

[Samvidbuho]

Mobile phase A was H2O + 0.1% TFA,
Mobile phase B was methanol,
Ran gradient of 5% B to 50% B over 17.5 min.

Column was a Zorbax C18.

 

[Samvidbuho]

Thinking about it now, using an acidic mobile phase doesn't make sense, right?

 

[Samvidbuho]

From Clarke's Analysis of Drugs and Poisons,
The 3rd / last spectrum I posted looks like the reference one for N,N DMT..