Equipment
-1 medium or small crock-pot (for heating materials)
-1 mg digital scale (for weighing product)
-1 digital ph meter (for measuring acidification and basification)
-1 coffee-grinder (for grinding plant materials)
Supplies
-2 wide-bottom chianti bottles w/ rubber corks (as separatory funnels)
-4 large mason jars w/ lids (for mixing materials)
-1 five-gallon bucket w/ lid & stirring apparatus (for final collection and disposal of materials)
-2 rubber nasal aspirator (as transfer pipettes)
-1 shallow glass lid w/ side handles (as evaporation dish)
-appropriate lengths and sizes of vinyl tubing (for siphoning)
-appropriate amount of conical coffee-filters (drying and collecting product)
-1 pair of chemical resistant gloves (for chemical handling)
-3 measuring beakers (for freezer precipitation)
-1 Scraping Tool (for product collection)
-appropriate amounts of cotton "flour sacks" (for straining of plant materials)
Materials
-appropriate quantity of MHRB (mimosa hostilis root-bark)
-appropriate amount of jugs of distilled water (for use in all aqueous solutions)
-1 bottle of muriatic HCl acid (for acidification)
-1 bottle of lye NaOH crystals (for basification)
-1 can of VM&P naphtha (NPS, Non-Polar Solvent)
-1 bottle of distilled white vinegar (for neutralizing lye spills)
Procedure
1. Break up MHRB by hand, and pulverize with coffee grinder; collect powdered and shredded material.
2. Prepare a solution of HCl and water at Ph2 in jarA. (it won't take much HCl, dilute if Ph is too low)
3. Combine pulverized MHRB and HCl solution in jarB so that MHRB is completely submerged.
4. Heat jarB in crock-pot with water for at least 30min, remove and leave overnight, shaking contents occasionally in meantime. (alkaloids will be converted into a water-soluble HCl salt-form)
5. Strain material with flour sacks, and collect the resulting aqueous solution in jarC.
[Save MHRB material and repeat steps 2-5 two more times, for a total of three HCl extractions, but do not strain on third extraction]
6. Prepare a solution of NaH and water in jarD to a Ph slightly greater than 12. (be careful, lye can be dangerous and the jar will heat up, so mix slowly)
7. In jarC, premix a slightly less than adequate amount of NPS with resulting solution in order to maximize the contact between the two solutions.
[On the third extraction, the MHRB material will remain in the solution until transfer to separatory funnel]
8. Before the NPS and aqueous solution separate, add NaOH solution until Ph is 12 or slightly higher. (product will be rendered as freebase and will become more soluble in NPS)
9. Stir contents to satisfaction.
10. Siphon contents of jarC into an empty distilled water jug, and shake jug for a more adequate mix.
11. Pour or siphon contents into separatory funnel.
12. As separation occurs and emulsions subside remove NPS layer with siphon tubes and/or transfer pipettes from the bottle neck while transferring enough solution between bottles to force the top layer further up the bottle neck.
[The easiest way to collect all NPS into one separatory funnel is to fix vinyl tubing tightly onto a nasal aspirator in order extend the pipette's reach; this transfer can be done with emulsions]
[NPS can be pulled off the top of emulsions and collected as emulsions settle while avoiding emulsion contamination in beakers.]
13. Collect NPS layer containing product in beakers, cover with tin-foil, keeping each of the three different pulls separate in order to gauge results.
14. Place beakers in freezer, properly saturated solutions should appear milky within an hour; if so, leave in freezer to form crystals until solution is clear (unsaturated).
[NPS can be reused if unsaturated in attempt to pull more product, and if it remains unsaturated, evaporate in evaporation dish sitting over-top crock-pot so that the steam heats the dish.]
[Continue pulling product with unsaturated NPS until until resulting solution is unsaturated.]
[Save aqueous solution w/ MHRB material in five-gallon bucket for final extractions and eventual disposal]
15. Scrape crystals into conical filter while still cold and allow to dry.
16. Collect product in sealed vial, store away from light, heat and moisture.
Administration
-1 Glass Medicine dropper w/ rubber removed
-1 Butane Torch Lighter
-1 Stainless Steel Spring
-1 Copper Scrubbing Mesh
-1 Pair Scissors
1. Cut a piece of copper mesh to a length between 1/4 and 1/3 the length of the dropper tube.
2. Cut a piece of spring long enough to fill the rest of the tube while leaving at least an eighth of the length open.
3. Burn both metal pieces until no more smoke is emitted.
4. Place spring in the tube followed by the mesh leaving the last eighth on the wide end open.
5. Test air passage.
6. Load product into the open end and heat carefully in short blasts in order to melt product into the mesh.
7. Heat the wide end indirectly while slowly inhaling through the narrow end; take between 3 and 4 tokes, holding them in long enough so as not to exhale vapor.
8. Blast off.
-1 medium or small crock-pot (for heating materials)
-1 mg digital scale (for weighing product)
-1 digital ph meter (for measuring acidification and basification)
-1 coffee-grinder (for grinding plant materials)
Supplies
-2 wide-bottom chianti bottles w/ rubber corks (as separatory funnels)
-4 large mason jars w/ lids (for mixing materials)
-1 five-gallon bucket w/ lid & stirring apparatus (for final collection and disposal of materials)
-2 rubber nasal aspirator (as transfer pipettes)
-1 shallow glass lid w/ side handles (as evaporation dish)
-appropriate lengths and sizes of vinyl tubing (for siphoning)
-appropriate amount of conical coffee-filters (drying and collecting product)
-1 pair of chemical resistant gloves (for chemical handling)
-3 measuring beakers (for freezer precipitation)
-1 Scraping Tool (for product collection)
-appropriate amounts of cotton "flour sacks" (for straining of plant materials)
Materials
-appropriate quantity of MHRB (mimosa hostilis root-bark)
-appropriate amount of jugs of distilled water (for use in all aqueous solutions)
-1 bottle of muriatic HCl acid (for acidification)
-1 bottle of lye NaOH crystals (for basification)
-1 can of VM&P naphtha (NPS, Non-Polar Solvent)
-1 bottle of distilled white vinegar (for neutralizing lye spills)
Procedure
1. Break up MHRB by hand, and pulverize with coffee grinder; collect powdered and shredded material.
2. Prepare a solution of HCl and water at Ph2 in jarA. (it won't take much HCl, dilute if Ph is too low)
3. Combine pulverized MHRB and HCl solution in jarB so that MHRB is completely submerged.
4. Heat jarB in crock-pot with water for at least 30min, remove and leave overnight, shaking contents occasionally in meantime. (alkaloids will be converted into a water-soluble HCl salt-form)
5. Strain material with flour sacks, and collect the resulting aqueous solution in jarC.
[Save MHRB material and repeat steps 2-5 two more times, for a total of three HCl extractions, but do not strain on third extraction]
6. Prepare a solution of NaH and water in jarD to a Ph slightly greater than 12. (be careful, lye can be dangerous and the jar will heat up, so mix slowly)
7. In jarC, premix a slightly less than adequate amount of NPS with resulting solution in order to maximize the contact between the two solutions.
[On the third extraction, the MHRB material will remain in the solution until transfer to separatory funnel]
8. Before the NPS and aqueous solution separate, add NaOH solution until Ph is 12 or slightly higher. (product will be rendered as freebase and will become more soluble in NPS)
9. Stir contents to satisfaction.
10. Siphon contents of jarC into an empty distilled water jug, and shake jug for a more adequate mix.
11. Pour or siphon contents into separatory funnel.
12. As separation occurs and emulsions subside remove NPS layer with siphon tubes and/or transfer pipettes from the bottle neck while transferring enough solution between bottles to force the top layer further up the bottle neck.
[The easiest way to collect all NPS into one separatory funnel is to fix vinyl tubing tightly onto a nasal aspirator in order extend the pipette's reach; this transfer can be done with emulsions]
[NPS can be pulled off the top of emulsions and collected as emulsions settle while avoiding emulsion contamination in beakers.]
13. Collect NPS layer containing product in beakers, cover with tin-foil, keeping each of the three different pulls separate in order to gauge results.
14. Place beakers in freezer, properly saturated solutions should appear milky within an hour; if so, leave in freezer to form crystals until solution is clear (unsaturated).
[NPS can be reused if unsaturated in attempt to pull more product, and if it remains unsaturated, evaporate in evaporation dish sitting over-top crock-pot so that the steam heats the dish.]
[Continue pulling product with unsaturated NPS until until resulting solution is unsaturated.]
[Save aqueous solution w/ MHRB material in five-gallon bucket for final extractions and eventual disposal]
15. Scrape crystals into conical filter while still cold and allow to dry.
16. Collect product in sealed vial, store away from light, heat and moisture.
Administration
-1 Glass Medicine dropper w/ rubber removed
-1 Butane Torch Lighter
-1 Stainless Steel Spring
-1 Copper Scrubbing Mesh
-1 Pair Scissors
1. Cut a piece of copper mesh to a length between 1/4 and 1/3 the length of the dropper tube.
2. Cut a piece of spring long enough to fill the rest of the tube while leaving at least an eighth of the length open.
3. Burn both metal pieces until no more smoke is emitted.
4. Place spring in the tube followed by the mesh leaving the last eighth on the wide end open.
5. Test air passage.
6. Load product into the open end and heat carefully in short blasts in order to melt product into the mesh.
7. Heat the wide end indirectly while slowly inhaling through the narrow end; take between 3 and 4 tokes, holding them in long enough so as not to exhale vapor.
8. Blast off.