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dmt in shroom substrate

Migrated topic.
I wonder if theis process could also be applied to 5-ht? Jeeze, some one has to test all of these someday. I haven't heard reports on many, if any 4-ho, 4-po tryptamines other than psilocin and psyclobin (sp's?)
 
DMT is good for expanding mushroom or LSD trips. I prefer smokin DMT 2-3 hours before droppings acid or eating mushrooms. works untill the next day i guess.... :D very intense CEVs i experienced
 
Does anyone have this paper available? Gartz demonstrates that P. cubensis produced high amounts of 4-HO-DET and minor amounts of 4-PO-DET from adding N,N-diethyl-tryptamine to the mycelial culture.
 
Great, thank you! No one is able to repeat it, you say? Are we sure that these repetitions are done under the exact same conditions and the exact same way as Gartz? Do you by any chance have any paper available where there is attempted to repeat the experiment?
 
i've searched for such papers, but have yet to find one.
and the folks over at the shroomery who speak of doing the experiments themselves never report any results.

genetics is always a factor in these experiments, as are the growth parameters. some strains may have better expression of certain enzymes than others; thus, concentration of solutions would need to be adjusted accordingly. conc. is obviously very important as well
 
Fuego said:
whats the deal with this stuff... good information is hard to come by, and i havnt found any solid reports of people trying this. any information would be apreciated.

think for every hit of dmt you have having two trips of shrooms (50mg dmt --> 25mg psilocybin/psilocin X 2) i would say thats pretty fantastic, dont you agree?
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i have tried my hand at this before with much success... BUT the dmt must be in the substrate from the very beginning or your precious dmt will be wasted. i dissolved the dmt freebase in everclear and soaked it all up in a pf cake. casing methods with dmt in the substrate was less successful for me. i sterilized the cakes in a pot of boiling water careful to kill germs, but not kill dmt. 1.5g was ridiculous.

edit---also check this out: Shroomery - A Successful Dream: Increasing the Potency of P. Cubensis
 
Let me see if I understand what you are saying.

You took DMT freebase and dissolved it in alcohol then let your PF cake absorb the DMT laden alcohol?

I've spilt a bit of alcohol on both a cake and a casing before and neither of them ended up very well. The mycelium died wherever the alcohol hit it.

Dunno, I'm glad it worked for you, but I think it would end up killing the cakes. Wouldn't it just be easier to convert to a salt, neutralize, and let the cake soak up the neutral pH water?
 
Maybe he ment that he soaked the substrate before adding the spores, ensuring that the alcohol was evaporated when the mycellium growth began?
 
Jeah ... he must have added the Ethanole before sterilizing , and even before moistening the substrate with water.
Alcohol kills the mushroom -small Amounts cause Mutant Shrooms .
 
What would you assume to be the best way to go about conducting an experiment on this? SWIM was thinking of inoculating a few jar with some DMT fumarate, do you think this would work? Should he instead try the alcohol soak method? He might try both. He was gunna use some pure white spice, but he also considered trying one with some jungle. How many mgs do you think would be needed to add to the substrate and what would be the best method of adding the spice?
 
mattritt said:
What would you assume to be the best way to go about conducting an experiment on this? SWIM was thinking of inoculating a few jar with some DMT fumarate, do you think this would work? Should he instead try the alcohol soak method? He might try both. He was gunna use some pure white spice, but he also considered trying one with some jungle. How many mgs do you think would be needed to add to the substrate and what would be the best method of adding the spice?
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Well DMT fumarate is soluable in water, so you could just add some to the water you use when making up the substrate. How much I don't really have a good feel for. Maybe try several different amounts ranging from 0.01 to 1% by weight of the water?

elphologist
 
how bout instead of putting dmt freebase or salt in to the substrate, try this instead:

pour off a little bit of an aya brew into a bottle,

use to feed substrate thru growth myceliunm stage/growth cycle...

im sure something nice would happen, and i guess everytime you cahnge out the water you could spray a little more aya, even if you dilute it a whole bunch... might be nice..

one could also try adding phalaris clippings and certain leaves to the substrate mix.. might be pretty cool.
 
Sorry to bring up old topic, but just found this:

However there is a very interesting study that took place in Leipzig about 15 years ago. Jochen Gartz, a mushroom explorer whom I know quite well, has done some fascinating studies with Psilocybe species by raising them on solid media containing strange tryptamines that are alien to the mushroom. Apparently the enzymes that are responsible for the 4-hydroxy group of psilocin are indifferent to what it is they choose to 4-hydroxylate. He has taken things like DPT or DIPT and put them in the growth media and the fruiting bodies that came out contain 4-hydroxy-DPT or 4-hydroxy-DIPT instead of psilocin. In fact, he has a patent on the process. These active compounds are made by the mushroom so they really are natural and yet they never have been observed in nature. I'll give you even odds that if you put spores of a psilocybe species on cow droppings loaded with 5-MeO-DMT you would come out with mushrooms containing 4,5-HO-MeO-DMT. This way you avoid a 10 step synthesis by growing a psychoactive mushroom that contains no illegal drug.
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That is Alexander Shulgin talking....sooooo...................

Also, link to here I found that here.
 
Another approach would be a simple Limetek; and then after pulling with D-limo, salt with vinegar, and use that solution to hydrate the perlite component of a PF tech.:idea:
 
plumsmooth said:
Another approach would be a simple Limetek; and then after pulling with D-limo, salt with vinegar, and use that solution to hydrate the perlite component of a PF tech.:idea:
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....*vermiculite component

And this might make the substrate more acidic...? We usually want to make it slightly basic if anything, so that nasty growths are discouraged and our intended cultures can still grow.
 
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