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Ever wonder whats in your peyote extracts? GC-MS analysis

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burnt

burnt

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SWIMs imaginary friend had some extra time and decided to write a theoretical report:

The details of extraction will not be gone into however peyote was extracted with traditional A/B method. The non polar solvent used was either toluene or xylene and the freebase was made into a salt with HCl.

So what is actually in this salt powder?

Here is a theoretical GC chromatogram
 

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Now what about the other two peaks?

The first small one at 16.3 minutes is theoretically Phenethylamine, 3,4,5,-trimethoxy-alpha-methyl. Interesting compound any idea on its activity? I think it might be in PHIKAL.

Below in green is the referance spectrum and structure. And in red is the spectrum aquired. They match pretty good except the 44 is missing but that is a result of theoretically mass spectrum aquisition conditions (what I mean is the theoretical instrument does not measure ions that low for sensitivity reasons). But then again this is just an MS spectrum and the confirmation is not known with certainty. That would require NMR (edited this in as its true).
 

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And just for fun here is what a chromatogram of the xylene extract theoretically looks like concentrated.

You see that the unknown compound is the major peak. Interesting that this compound does not go into salt form as easily as mescaline perhaps its not an alkaloid? There are also two peaks in the beginning which are unknown.
 

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To conclude I hope you have enjoyed this theoretically made up report of what kind of compounds are present in a peyote extract after A/B then HCl salting. This goes to show that when you take a plant extract you are injesting more then one compound that may be active, which is why the effects vary from pure synthetic compounds.
 
burnt said:
To conclude I hope you have enjoyed this theoretically made up report of what kind of compounds are present in a peyote extract after A/B then HCl salting. This goes to show that when you take a plant extract you are injesting more than one non-alkaloidal compound that may be active, which is why the effects vary from pure synthetic compounds.
Click to expand...
I may add
 
True but there are definately two alkaloids here. And one compound most likely non alkaloidal. Another hint that the 3rd peak is not alkaloidal is the mass is even so it probably does not contain nitrogen unless it has two nitrogens.

MSDS Phenethylamine, alpha-methyl-3,4,5-trimethoxy-,hydrochloride CAS 5688-80-2 MSDS * Amphetamine, 3,4,5-trimethoxy-, hydrochloride * Benzeneethanamine, 3,4,5-trimethoxy-alpha-methyl-, hydrochloride * alpha-Methyl-3,4,5-trimethoxyphenethylamine hydr

CAS 5688-80-2 Phenethylamine, alpha-methyl-3,4,5-trimethoxy-,hydrochloride * Amphetamine, 3,4,5-trimethoxy-, hydrochloride * Benzeneethanamine, 3,4,5-trimethoxy-alpha-methyl-, hydrochloride * alpha-Methyl-3,4,5-trimethoxyphenethylamine hydrochloride * Phenethylamine...
www.chemcas.com

This is the only report that could be found concerning the activity of Phenethylamine, 3,4,5,-trimethoxy-alpha-methyl
 
I would bet that that compound is actually N-Methyl-mescaline. The amphetamine compounds have yet to be found in cacti, the N-methyls are well known in the literature.

GREAT WORK.
Now lets think about that other peak. 2 N does seem unlikely though?????

I guess your friend's extraction tech excluded the phenolic compounds. These are also prevalent in the cacti. but would require a diff tech to isolate, although you could likely pull them by acidifying the basic solution, once the goods have been removed, and extracting with a solvent. But you may get some crap too, so a further purification would be necessary.
 
Thanks a lot for pointing that out. SWIM is not knowledgable on the other cactus alkaloids. Also didn't realize this compound was TMA. SWIM was scouring through Pihkal looking for it but shulgins naming system is sometimes tricky. hehe

As far as the third peak SWIM can look through his friends notes and post some of the library matches. They looked weird though which is normal with these libraries sometimes you just get weird results.
 
Yes SWIM will get some more info on monday. In the concentrated toluene (or xylene SWIM can't remember) layer he can also look at some of the minor peaks and see what comes up.

SWIMs friend is thinking about screening some cacti species using this instrument but needs time before that data is presented. Also wants to do this with some more dmt plants but needs more samples and time aaaaaah so much to do so little time...
 
Very interesting! This shows how mescaline is only one of the compounds that effects you while intoxicated on the Sacred Cactus!
 
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