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Experimental Ergoline-Alkaloid Extractions (everything about LSA's)

Migrated topic.
scrolling down through the link, you'll find another link

but be advised, it's considered hazardous material.
while c. paspali isn't as toxic as c. purpurea, it's been shown to be toxic to bovine when consumed in large amounts. the other issue is the tendency for spores to spread and infect local crops.

the best option to get the strain is to scour the grass for it, and transfer to agar plates. the grass (paspalum dilatatum) grows just about everywhere in temperate regions.
one who is equipped with a makeshift glovebox and petri plates should be able to isolate a pure strain, once a raw one is obtained.



or you could just go the safe route, and get a bunch of HBRW seeds.
 
tjamks a lot for the infos benzyme swim will check fields this early spring
who finds a paspali sclerotia has found a treasure! 😉
 
benzyme said:
Elf Machine said:
There was obviously very little if any conversion of LSA to LSH.
Click to expand...


of course not... why would there be?

HBWR produces LSH, so does C. Paspali.
the latter produces LSH at an avg. of 1.2 g per liter of culture. that's about the same yield as a kg of HBWR.
Click to expand...


The Elf doesn't understand. He was under the impression the LSA could possibly be converted to LSH by the acetaldehyde in the rum and peppermint. He assumed this from some of 69ron's posts. Is the Elf out to lunch?
 
Elf Machine said:
The Elf doesn't understand. He was under the impression the LSA could possibly be converted to LSH by the acetaldehyde in the rum and peppermint. He assumed this from some of 69ron's posts. Is the Elf out to lunch?
Click to expand...
Let alone that this conversion is hypothetical, 69ron is really not very credible in what he claims. Don't take his words for granted!
 
benzyme said:
here's the 1961 paper by Arcamone et al.

(KH2PO4 is potassium dihydrogen phosphate, or monopotassium phosphate, commonly used to prepare pH buffer solution.
MgSO4 * 7H2O is magnesium sulfate heptahydrate, aka epsom salts)
Click to expand...

I want to see the 500 liter stainless steel vessel once you acquire it..... now that you have the small fermenter. These guys were after some serious amounts of LSH given that the abstract states that the yield of LSH is 1 mg per ml. With 300 liters of medium in that vessel you'd get 300 grams of LSH. Hmmmmmm....
 
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