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from about 50 - 100 mg dry p.brachystachys
- Thread starter benzyme
- Start date
bioassay?
actualfactual
Rising Star
nice
Shadowman-x said:
none yet. opted to do lc-mc analysis on it first
hmm...base peak is 263 m/z, and broad (sample too concentrated)
very odd :?
no idea what that is. this will take some sleuthing
very odd :?
no idea what that is. this will take some sleuthing
be careful..ive played with grass and instead of DMT, I had weird alks that made me sick..tryptamine feeling with mild visuals but whateer else was there was not nice.
fractal enchantment said:
duly noted
i'll work on a simple sep tek once I can identify the alks present
Very nice to see this kind of progress, greatly appreciated :d
if you orders these seeds, whatever you do...don't throw them outside in the soil
this is a very invasive species
this is a very invasive species
so, how it was done:
-p. brach seeds were grown in potting mix with coir and 100 mL 10 mM tryptamine hcl solution
added.
-dried grass was sonicated for 5 mins in 45 mL household vinegar (pH ~ 2.6),
-the slurry was frozen, thawed, sonicated again.
-Vac filtration, and the acidic aqueous phase was saved. this was basified with 6 N NaOH up to a pH of 12.56.
-added approx 10 mL methylene chloride (Me2Cl2) to the basic phase, inverting the sep funnel several times
-poured off the Me2Cl2 in an 8 oz jam jar and evap'ed at RT.
(notes: simmering the plant material slurry for an hour or more would work as effectively as sonication. heating up the acidic phase will catalyze [hydro]lysis on the plant cell walls. warm naptha may be subbed for Me2Cl2, do at least 3 pulls.)
after transferring a bit into a microcentrifuge tube with a toothpick, swim would estimate around 10 mg is on the bottom of a jar. he wanted to demonstrate that this is an alk-rich alternative to traditional sources.
-p. brach seeds were grown in potting mix with coir and 100 mL 10 mM tryptamine hcl solution
added.
-dried grass was sonicated for 5 mins in 45 mL household vinegar (pH ~ 2.6),
-the slurry was frozen, thawed, sonicated again.
-Vac filtration, and the acidic aqueous phase was saved. this was basified with 6 N NaOH up to a pH of 12.56.
-added approx 10 mL methylene chloride (Me2Cl2) to the basic phase, inverting the sep funnel several times
-poured off the Me2Cl2 in an 8 oz jam jar and evap'ed at RT.
(notes: simmering the plant material slurry for an hour or more would work as effectively as sonication. heating up the acidic phase will catalyze [hydro]lysis on the plant cell walls. warm naptha may be subbed for Me2Cl2, do at least 3 pulls.)
after transferring a bit into a microcentrifuge tube with a toothpick, swim would estimate around 10 mg is on the bottom of a jar. he wanted to demonstrate that this is an alk-rich alternative to traditional sources.
Really great work man, fantastic! Await the results with much interest.
so what can be the 263 peak, still no idea?
all advances in this area are very appreciated, thanks for the work benz!
all advances in this area are very appreciated, thanks for the work benz!