When I poured out the acid tea into a pot, I didn't filter it properly. I just used a normal kitchen sieve, with no muslin cloth. I think that could be reason why it screwed up here.
When I poured out the solvent after the freeze precipitation step, this is what I saw. Notice how it's all one solid. It was a frozen block of something. I let it melt, and froze it again. After each freeze step, I poured out the solvent. I transferred what was left into a Falcon tube and added fresh solvent to resuspend it. I let it settle, and there were 2 distinct layers, with the bottom layer containing a lot of sediment.
There is also a milky precipitate in the solvent layer.
My question is what did I do wrong and can I make use of the mess I made? I'm predicting that I didn't filter it out properly, and I left some of that basified tea in the freeze precip container.
When I poured out the solvent after the freeze precipitation step, this is what I saw. Notice how it's all one solid. It was a frozen block of something. I let it melt, and froze it again. After each freeze step, I poured out the solvent. I transferred what was left into a Falcon tube and added fresh solvent to resuspend it. I let it settle, and there were 2 distinct layers, with the bottom layer containing a lot of sediment.
There is also a milky precipitate in the solvent layer.
My question is what did I do wrong and can I make use of the mess I made? I'm predicting that I didn't filter it out properly, and I left some of that basified tea in the freeze precip container.
