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LSASA + TASA tek

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mew

mew

huachumancer
im sure i read something along these lines somewhere around these parts but after searching a while i cant find it so, heres what i hope to try, please let me know if theres a snag in the theory.


hbwr are powdered and immediately submerged into dry acetone
several acetone pulls are performed
combine all acetone pulls and filter
decant filtered acetone
prepare tartrate acid saturated acetone (tasa)
combine hbwr acetone pulls with tasa
freeze acetone in clear glass to observe precipitation
once precipitates settled decant away from the LSA tartrate
when this dries the LSA tartrate will have minimal tartaric acid contamination


during this process avoidance of sunlight and high temperatures will be observed
lsa tartrate will be stored in an airtight, cold, lightless place
 
rather than bassifying to ph 9 i assume that the acetone will dissolve the lsa in its natural form (as previous acetone pulls yielded active yellow goo) and with the addition of acid in acetone the lsa will be converted to tartrate salt

this process ignores defats as well considering the product is freeze precipitated and will most likely be free from oils and undesirable contamination
 
it seems the most ideal solvent to use, however given that i am not able to access ethyl acetate without ordering it specifically (which i wont do), unless i am mistaken i will proceed with acetone.

back to my original query, is the outlined method functional in a theoretical perspective.

lastly, what might be the necessary temperature for freeze precipitation/ time needed.

an innovation struck me, rather than freeze precipitate, ill evaporate the TASA+LSASA in a cool dark place to yield LSA tartrate+ tartaric acid. which can be washed with minimal dry cold acetone to remove excess acid, does this seem feasible?
 
I got 8.5g of morning glory seeds from my garden and played with them a bit last night:

- Ground seeds (powder was grey), pulled with ethyl acetate (solvent became yellowish), added a pinch of citric acid powder, nothing crashed.
- Added some water and lime to turn the powder into a paste which became yellow, pulled again with ethyl acetate, added citric, no precipitation.
- Finally, pulled with acetone. Added citric acid, clouding and precipate formed (promising?). Decanted and rinsed with acetone once. Dissolved precipitate in water, decanted onto drying dish leaving some stuff that didn't dissolve behind. Residue from drying the water is crystalline off-white and weighed 12mg (see picture). Could this be LSA citrate?

I guess I can bioassy 4mg or so, see how it goes. If active perhaps I can send it to Benz to check out.

Assuming the mono salt form, this is 0.08% yield. Literature has 0.06% which is consistent. 6mg of LSA is considered an active dose (paper attached).
 

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I'm curious about where you are going with this Loveall. I have a few plants I'm about to harvest pretty soon and would love to know what you think before proceeding.
 
Loveall said:
- Ground seeds (powder was grey), pulled with ethyl acetate (solvent became yellowish), added a pinch of citric acid powder, nothing crashed.
- Added some water and lime to turn the powder into a paste which became yellow, pulled again with ethyl acetate, added citric, no precipitation.
- Finally, pulled with acetone. Added citric acid, clouding and precipate formed (promising?). Decanted and rinsed with acetone once. Dissolved precipitate in water, decanted onto drying dish leaving some stuff that didn't dissolve behind. Residue from drying the water is crystalline off-white and weighed 12mg (see picture). Could this be LSA citrate?
Click to expand...

Hi Loveall, is my understanding correct: simply mix ground seeds with acetone, filter off the acetone, and add citric acid?

Or is the CaOH past necessary before pulling with acetone?
 
Gave your version a go Loveall,


-ground seeds
-based with lime water
-pulled with EA
-salted with citric acid
-decanted, dried, collected with H20 and evaporated.
Ended up with a dark citrate goo.

2nd pull I used acetone and salted with citric acid. A white precipitation has crashed, unsure how easy it'll be to decant/ filter.

Hoping to do the acetone run again with a larger batch. Will keep ya posted.

Edit: got contamination results are totally unreliable.
 
Hello all!

Reviving this post after an year.

Hey Loveall and Trip! Had any chance to try your precipitates? Any tests done on them? At least some UV-fluor of it?
 
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